María Cruz Miraglia, Melanie Barrios-Benito, Sabrina Galdo-Novo, Danilo Bucafusco, Ana Taffarel, Alejandra Victoria Capozzo, Manuel Victor Borca, Daniel Mariano Pérez-Filgueira
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Serum samples were collected up to 70 dpv and tested by a virus-neutralizing test against serotype O strains from a South American lineage and two strains representative of two Asian lineages. Our results showed that vaccines containing the ME-SA topotype O1/Campos strain developed cross-neutralizing responses against the two Asian viruses after the first vaccination. In contrast, significant heterotypic neutralizing antibody titers against the homologous topotype strain were only found after the second vaccination, indicating that the phylogenic relationship may differ from the antigenic profiles for these two viruses. The amount of the O1/Campos strain and the revaccination were essential factors for neutralization against the homologous- and heterologous-type O FMDV viruses. 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引用次数: 0
摘要
口蹄疫病毒(FMDV)的抗原变异性很高,这给制定预防策略带来了挑战,强调了研究针对一系列病毒株提供广泛保护的疫苗的必要性。本文研究了针对血清型 O 株的不同疫苗方案的异型交叉反应,评估了再接种、抗原剂量和加入其他 FMDV 血清型的影响。用七种不同的 FMDV 疫苗对初免牛进行免疫接种,分别在初免后 0 天、28 天和 56 天接种三剂相同配方的疫苗。收集血清样本至 70 dpv,并通过病毒中和试验对来自南美的血清型 O 株和来自亚洲的两个血清型代表株进行检测。我们的结果表明,含有 ME-SA 拓扑 O1/Campos 株的疫苗在首次接种后会产生针对两种亚洲病毒的交叉中和反应。相反,只有在第二次接种后才会发现针对同源顶型毒株的明显异型中和抗体滴度,这表明这两种病毒的系统发育关系可能与抗原谱不同。O1/Campos 株的数量和再次接种是中和同源和异源 O 型口蹄疫病毒的关键因素。疫苗的毒株组成只与针对其中一种亚洲毒株的交叉中和有关,这表明这种模式可能存在亚型内分化。
Impact of Different Foot and Mouth Disease Vaccine Schemes in Cross-Neutralization Against Heterologous Serotype O Strains in Cattle.
The high antigenic variability of the foot-and-mouth disease virus (FMDV) represents a challenge for developing prophylactic strategies, stressing the need for research into vaccines offering broad protection against a range of virus strains. Here, the heterotypic cross-reaction using different vaccine schemes against serotype O strains was studied, evaluating the impact of revaccination, antigen dose, and incorporation of additional FMDV serotypes. Naïve cattle were immunized with seven distinct FMDV vaccines, receiving three doses of the same formulation at 0, 28, and 56 days post-primary vaccination (dpv). Serum samples were collected up to 70 dpv and tested by a virus-neutralizing test against serotype O strains from a South American lineage and two strains representative of two Asian lineages. Our results showed that vaccines containing the ME-SA topotype O1/Campos strain developed cross-neutralizing responses against the two Asian viruses after the first vaccination. In contrast, significant heterotypic neutralizing antibody titers against the homologous topotype strain were only found after the second vaccination, indicating that the phylogenic relationship may differ from the antigenic profiles for these two viruses. The amount of the O1/Campos strain and the revaccination were essential factors for neutralization against the homologous- and heterologous-type O FMDV viruses. The strain composition of the vaccine was only relevant for cross-neutralization against one of the Asian strains, suggesting potential intra-serotypic divergences for this pattern.
期刊介绍:
Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.