{"title":"苏丹 Naja nubiae(蛇类:Elapidae)毒液的神经毒性效应。","authors":"Huda Khalid, Maowia M Mukhtar","doi":"10.1093/trstmh/trae116","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Neurotoxicity is a common feature of elapid snake envenomation. There are limited studies on the toxicity of Naja nubiae venom, the Nubian spitting cobra, from north-east Africa.</p><p><strong>Methods: </strong>We used the chick biventer cervicis nerve-muscle preparation to demonstrate the neurotoxic effect of N. nubiae venom and to compare it with the potent neurotoxic cobra Naja melanoleuca venom. Venoms were separated by successive reverse-phase high-performance liquid chromatography (RP-HPLC) runs and the molecular mass of the neurotoxins was determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).</p><p><strong>Results: </strong>Both venoms caused time-dependent inhibition of nerve-mediated twitches with a t90 value of 22.2±1.9 min and 12.9±1.2 min for N. nubiae and N. melanoleuca venoms, respectively. Prior incubation of some commercial antivenom (EchiTab-Plus-ICP [Costa Rica], CSL, Parkville, Victoria, Australia) and snake venom antisera [India]) did not prevent the neurotoxic effect of N. nubiae venom. The chromatographic separation of N. nubiae and N. melanoleuca venoms followed by MALDI-TOF MS analysis revealed that short-chain α-neurotoxin accounted for 8.4% of N. nubiae and 14.8% of N. melanoleuca whole venoms.</p><p><strong>Conclusions: </strong>N. nubiae venom, which was previously known as cytotoxic venom, exhibits considerable in vitro neurotoxic effects on chick nerve-muscle preparations that may have consequences for antivenom development in north-east Africa.</p>","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The neurotoxic effect of Naja nubiae (Serpentes: Elapidae) venom from Sudan.\",\"authors\":\"Huda Khalid, Maowia M Mukhtar\",\"doi\":\"10.1093/trstmh/trae116\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Neurotoxicity is a common feature of elapid snake envenomation. There are limited studies on the toxicity of Naja nubiae venom, the Nubian spitting cobra, from north-east Africa.</p><p><strong>Methods: </strong>We used the chick biventer cervicis nerve-muscle preparation to demonstrate the neurotoxic effect of N. nubiae venom and to compare it with the potent neurotoxic cobra Naja melanoleuca venom. Venoms were separated by successive reverse-phase high-performance liquid chromatography (RP-HPLC) runs and the molecular mass of the neurotoxins was determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).</p><p><strong>Results: </strong>Both venoms caused time-dependent inhibition of nerve-mediated twitches with a t90 value of 22.2±1.9 min and 12.9±1.2 min for N. nubiae and N. melanoleuca venoms, respectively. Prior incubation of some commercial antivenom (EchiTab-Plus-ICP [Costa Rica], CSL, Parkville, Victoria, Australia) and snake venom antisera [India]) did not prevent the neurotoxic effect of N. nubiae venom. 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引用次数: 0
摘要
背景:神经毒性是伶蛇类中毒的常见特征。有关非洲东北部努比亚吐丝眼镜蛇 Naja nubiae 毒液毒性的研究十分有限:方法:我们使用小鸡双颈神经肌肉制剂来证明 N. nubiae 毒液的神经毒性作用,并将其与具有强烈神经毒性的眼镜蛇 Naja melanoleuca 毒液进行比较。毒液通过连续反相高效液相色谱法(RP-HPLC)分离,神经毒素的分子质量通过基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF MS)测定:结果:N. nubiae和N. melanoleuca两种毒液对神经介导的抽搐均有时间依赖性抑制作用,t90值分别为22.2±1.9 min和12.9±1.2 min。事先孵育一些商用抗蛇毒血清(EchiTab-Plus-ICP [哥斯达黎加],CSL,Parkville,Victoria,澳大利亚)和蛇毒抗血清 [印度])并不能阻止 N. nubiae 毒液的神经毒性作用。通过对努比亚蛇毒和黑斑鳢蛇毒进行色谱分离和 MALDI-TOF MS 分析,发现努比亚蛇毒和黑斑鳢蛇毒中的短链 α-神经毒素分别占整个蛇毒的 8.4% 和 14.8%:结论:N. nubiae毒液以前被称为细胞毒性毒液,在体外对小鸡神经肌肉制剂表现出相当大的神经毒性作用,这可能会对非洲东北部的抗蛇毒血清开发产生影响。
The neurotoxic effect of Naja nubiae (Serpentes: Elapidae) venom from Sudan.
Background: Neurotoxicity is a common feature of elapid snake envenomation. There are limited studies on the toxicity of Naja nubiae venom, the Nubian spitting cobra, from north-east Africa.
Methods: We used the chick biventer cervicis nerve-muscle preparation to demonstrate the neurotoxic effect of N. nubiae venom and to compare it with the potent neurotoxic cobra Naja melanoleuca venom. Venoms were separated by successive reverse-phase high-performance liquid chromatography (RP-HPLC) runs and the molecular mass of the neurotoxins was determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS).
Results: Both venoms caused time-dependent inhibition of nerve-mediated twitches with a t90 value of 22.2±1.9 min and 12.9±1.2 min for N. nubiae and N. melanoleuca venoms, respectively. Prior incubation of some commercial antivenom (EchiTab-Plus-ICP [Costa Rica], CSL, Parkville, Victoria, Australia) and snake venom antisera [India]) did not prevent the neurotoxic effect of N. nubiae venom. The chromatographic separation of N. nubiae and N. melanoleuca venoms followed by MALDI-TOF MS analysis revealed that short-chain α-neurotoxin accounted for 8.4% of N. nubiae and 14.8% of N. melanoleuca whole venoms.
Conclusions: N. nubiae venom, which was previously known as cytotoxic venom, exhibits considerable in vitro neurotoxic effects on chick nerve-muscle preparations that may have consequences for antivenom development in north-east Africa.
期刊介绍:
Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.