Su-Jin Shin, Inho Park, Heounjeong Go, Jiwon Ko, Yangkyu Lee, Jee Hung Kim, Sung Gwe Ahn, Joon Jeong, Soong June Bae, Yoon Jin Cha
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引用次数: 0
摘要
本研究探讨了三阴性乳腺癌（TNBC）和激素受体阳性、HER2 阴性乳腺癌（HR + HER2-BC）高 TIL（≥60%）病例中免疫细胞（IC）组成和空间分布的差异，重点关注 PD-L1 状态。通过对 18 例 TNBC 和 14 例 HR + HER2-BC 的切除肿瘤组织进行多重免疫荧光，我们分析了 IC 类型（CD20、CD8、CD4、FOXP3）及其空间相互作用。TNBC 表现出独特的 IC 构成，其特点是 CD8 + IC 比例较高（基质：27% vs 17%，p 2 vs 114.3 ± 146.9/mm2，p = 0.036），同时 IC 在 TC 附近明显聚集。根据 PD-L1 状态，两种肿瘤亚型的 IC 构成各不相同。总之，受PD-L1状态的影响，高TIL TNBC和HR + HER2-BC的IC组成和空间分布存在显著差异。
Immune environment of high-TIL breast cancer: triple negative and hormone receptor positive HER2 negative.
This study explores differences in immune cell (IC) composition and spatial distribution between triple-negative breast cancer (TNBC) and hormone receptor-positive, HER2-negative breast cancer (HR + HER2-BC) in high-TIL (≥60%) cases, focusing on PD-L1 status. Using multiplex immunofluorescence on resected tumor tissues from 18 TNBC and 14 HR + HER2-BC cases, we analyzed IC types (CD20, CD8, CD4, FOXP3) and their spatial interactions. TNBC showed a unique IC composition characterized by a higher proportion of CD8 + IC (stroma: 27% vs 17%, p < 0.001; tumor: 54% vs 31%, p < 0.001) and CD4 + FOXP3 + IC (stroma: 3.9% vs 3.0%, p = 0.036), compared to HR + HER2-BC. Notably, PD-L1 positive TNBC cases demonstrated denser infiltration CD4 + FOXP3 + IC in the stromal region compared to HR + HER2-BC (146.4 ± 67.1/mm2 vs 114.3 ± 146.9/mm2, p = 0.036), along with pronounced IC clustering near TC. Both tumor subtypes displayed varied IC compositions based on PD-L1 status. In conclusion, IC composition and spatial distribution in high-TIL TNBC and HR + HER2-BC significantly differ, influenced by PD-L1 status.
期刊介绍：
npj Breast Cancer publishes original research articles, reviews, brief correspondence, meeting reports, editorial summaries and hypothesis generating observations which could be unexplained or preliminary findings from experiments, novel ideas, or the framing of new questions that need to be solved. Featured topics of the journal include imaging, immunotherapy, molecular classification of disease, mechanism-based therapies largely targeting signal transduction pathways, carcinogenesis including hereditary susceptibility and molecular epidemiology, survivorship issues including long-term toxicities of treatment and secondary neoplasm occurrence, the biophysics of cancer, mechanisms of metastasis and their perturbation, and studies of the tumor microenvironment.
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