非靶向和靶向脂质组学揭示了 2 型糖尿病患者脂质代谢的动态变化。

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2024-11-10 DOI:10.3390/metabo14110610

Li Feng, Bingshu He, Jianzhen Xia, Zhonghua Wang

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引用次数: 0

摘要

背景：2 型糖尿病（T2DM）是一种复杂的代谢性疾病，越来越多的证据表明脂质代谢在其发病机制中起着核心作用。然而，人们对 T2DM 不同阶段脂质代谢的动态变化仍然缺乏研究：本研究旨在利用综合脂质组学方法阐明 T2DM 中脂质代谢的时间变化：方法：采用基于 LC-MS 的脂质组学方法，包括非靶向和靶向方法，对 155 名受试者的血清样本进行分析：结果：与健康对照组相比，我们在新诊断的 T2DM 患者中发现 44 种脂质代谢物发生了明显变化，在高危人群中发现 29 种脂质代谢物发生了明显变化。鞘磷脂、磷脂酰胆碱和甾醇酯代谢等关键代谢途径受到破坏，突出表明胰岛素抵抗和氧化应激参与了 T2DM 的进展。此外，13 种脂质代谢物具有诊断 T2DN 的潜力，随着病情的发展，这些代谢物呈现出一致的增加或减少趋势：我们的研究结果强调了脂质代谢在 T2D 发展过程中的重要性，并确定了用于早期诊断和监测疾病进展的潜在脂质生物标志物，这有助于为新型治疗策略铺平道路。

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Untargeted and Targeted Lipidomics Unveil Dynamic Lipid Metabolism Alterations in Type 2 Diabetes.

Background: Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder with a growing body of evidence suggesting the central role of lipid metabolism in its pathogenesis. However, the dynamic changes in lipid metabolism across different stages of T2DM remain understudied.

Objective: This study aimed to elucidate the temporal alterations in lipid metabolism in T2DM using an integrated lipidomics approach.

Method: Serum samples from 155 subjects were analyzed using LC-MS-based lipidomics, including untargeted and targeted approaches.

Results: We identified significant alterations in 44 lipid metabolites in newly diagnosed T2DM patients and 29 in high-risk individuals, compared with healthy controls. Key metabolic pathways such as sphingomyelin, phosphatidylcholine, and sterol ester metabolism were disrupted, highlighting the involvement of insulin resistance and oxidative stress in T2DM progression. Moreover, 13 lipid metabolites exhibited diagnostic potential for T2DN, showing consistent trends of increase or decrease as the disease progressed.

Conclusion: Our findings underscore the importance of lipid metabolism in T2D development and identify potential lipid biomarkers for early diagnosis and monitoring of disease progression, which contribute to paving the way for novel therapeutic strategies.

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.