基于基因组挖掘 Carpatamides I-M 及其候选生物合成基因簇。

IF 4.9 2区医学 Q1 CHEMISTRY, MEDICINAL

Marine Drugs Pub Date : 2024-11-20 DOI:10.3390/md22110521

Shu-Mei Shen, Yun-Chang Xie, Li-Rong Tu, Miao-Er Wu, Yan-Min Wang, Chun-Hui Song, Yu-Hui Sun, Ming-He Luo

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引用次数: 0

摘要

通过对源自海洋的副链霉菌 1268 进行化学研究，分离出了一种新的卡帕酰胺 I 化合物 (1)。随后的基因组分析确定了其候选生物合成基因簇 ctd，约 44 kb。为了获得更多的卡帕酰胺衍生物，我们对作为正调控因子的 Ctd14 进行了上调，获得了卡帕酰胺 I 的改进型和四种新的卡帕酰胺 J-M 化合物（2-5）。上述五种新分离物的结构是通过ESI-HRMS以及一维（1D）和二维（2D）光谱核磁共振数据集综合确定的。生物测定结果表明，1-5 号化合物对 A549、HT-29 和 HepG2 细胞系具有抗炎活性和微弱的细胞毒性。

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Genome-Based Mining of Carpatamides I-M and Their Candidate Biosynthetic Gene Cluster.

Chemically investigating the marine-derived Streptomyces parvus 1268 led to the isolation of a new compound of carpatamide I (1). Subsequent genomic analysis identified its candidate biosynthetic gene cluster ctd of approximately 44 kb. In order to obtain more carpatamide derivatives, we conducted the upregulation of Ctd14, which is a positive regulator, and obtained improvement of carpatamide I and four new compounds of carpatamides J-M (2-5). The structures of the aforementioned five new isolates were identified by a combination of ESI-HRMS as well as one-dimensional (1D) and two-dimensional (2D) spectral NMR datasets. Bioassay results showed that compounds 1-5 displayed anti-inflammatory activity and weak cytotoxicity against cell lines of A549, HT-29, and HepG2.

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来源期刊

Marine Drugs 医学-医药化学

CiteScore

9.60

自引率

14.80%

发文量

671

审稿时长

1 months

期刊介绍： Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.