Minji Choi, Jiyoung Kwon, Ji-Hyun Jang, Duck-Su Kim, Hyun-Jung Kim
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Cell viability was assessed using an MTT assay, cell migration was evaluated using wound healing and transwell assays, and osteogenic activity was determined through Alizarin Red S staining and a gene expression analysis of osteogenic markers (<i>ALP</i>, <i>RUNX-2</i>, <i>OCN</i>, and <i>Col1A2</i>). (3) Results: The combination of ELP and BAG significantly enhanced the viability of hPDLFs with an optimal BAG concentration of 1-4%. Cell migration assays demonstrated faster migration rates in groups with 2-4% BAG and ELP incorporation. Osteogenic activity was the highest with 2-3% BAG incorporation with ELP, as evidenced by intense Alizarin Red S staining and the upregulation of osteogenic differentiation markers. (4) Conclusions: The incorporation of ELP (organic) and BAG (inorganic) into HCSC significantly enhances the viability, migration, and osteogenic differentiation of hPDLFs. 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引用次数: 0
摘要
(1) 背景：本研究旨在通过加入有机和无机成分，特别是类弹性蛋白多肽（ELPs）和生物活性玻璃（BAG），来增强水门汀硅酸钙水门汀（HCSCs）的生物特性。我们重点研究了这些复合材料对人类牙周韧带成纤维细胞（hPDLFs）的活力、迁移和成骨分化的影响。 (2) 方法：在 Proroot MTA 中添加 1-5 wt% 63S BAG 和 10 wt% ELP。实验组包含 HSCS 与 ELP 和 BAG 的不同组合。细胞活力通过 MTT 试验进行评估，细胞迁移通过伤口愈合和跨孔试验进行评估，成骨活性通过茜素红 S 染色和成骨标志物（ALP、RUNX-2、OCN 和 Col1A2）的基因表达分析进行测定。(3）结果：ELP 和 BAG 的组合能显著提高 hPDLFs 的活力，最佳 BAG 浓度为 1-4%。细胞迁移试验表明，在 BAG 和 ELP 含量为 2-4% 的组中，细胞迁移率更快。BAG 与 ELP 结合浓度为 2-3% 时，成骨活性最高，这体现在强烈的茜素红 S 染色和成骨分化标志物的上调。(4）结论：在 HCSC 中加入 ELP（有机）和 BAG（无机）可显著增强 hPDLFs 的活力、迁移和成骨分化。这些研究结果表明，复合 HCSC 可支持牙髓病中破坏性骨病变的愈合。
Enhancing the Biological Properties of Organic-Inorganic Hybrid Calcium Silicate Cements: An In Vitro Study.
(1) Background: This study aimed to enhance the biological properties of hydraulic calcium silicate cements (HCSCs) by incorporating organic and inorganic components, specifically elastin-like polypeptides (ELPs) and bioactive glass (BAG). We focused on the effects of these composites on the viability, migration, and osteogenic differentiation of human periodontal ligament fibroblasts (hPDLFs). (2) Methods: Proroot MTA was supplemented with 1-5 wt% 63S BAG and 10 wt% ELP. The experimental groups contained various combinations of HSCS with ELP and BAG. Cell viability was assessed using an MTT assay, cell migration was evaluated using wound healing and transwell assays, and osteogenic activity was determined through Alizarin Red S staining and a gene expression analysis of osteogenic markers (ALP, RUNX-2, OCN, and Col1A2). (3) Results: The combination of ELP and BAG significantly enhanced the viability of hPDLFs with an optimal BAG concentration of 1-4%. Cell migration assays demonstrated faster migration rates in groups with 2-4% BAG and ELP incorporation. Osteogenic activity was the highest with 2-3% BAG incorporation with ELP, as evidenced by intense Alizarin Red S staining and the upregulation of osteogenic differentiation markers. (4) Conclusions: The incorporation of ELP (organic) and BAG (inorganic) into HCSC significantly enhances the viability, migration, and osteogenic differentiation of hPDLFs. These findings suggest that composite HCSC might support healing in destructed bone lesions in endodontics.
期刊介绍：
Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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