性别和早期生活压力体验对猪心脏和脑组织催产素和 H2S 系统表达的影响

IF 4.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Biomolecules Pub Date : 2024-10-30 DOI:10.3390/biom14111385
Franziska Münz, Nadja Abele, Fabian Zink, Eva-Maria Wolfschmitt, Melanie Hogg, Claus Barck, Josef Anetzberger, Andrea Hoffmann, Michael Gröger, Enrico Calzia, Christiane Waller, Peter Radermacher, Tamara Merz
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引用次数: 0

摘要

早期生活压力(ELS)会大大增加罹患慢性心血管疾病的风险,并可能引起神经炎症。这项事后研究基于之前一项显示雄性对照动物 "血清脑损伤标志物 "升高的研究材料,探讨了性别和/或 ELS 对 H2S 和催产素系统在大脑和心脏表达的影响。经图宾根地区委员会批准后,对 12 头性成熟、未阉割的德国大白猪进行了随机对照研究。对照组动物在 28-35 天时与母猪分离,而 ELS 组动物在第 21 天时与母猪分离。在 20-24 周时,对动物进行麻醉、通气和外科手术。对下丘脑、前额叶皮层和心肌组织样本进行催产素、催产素受体、H2S产生酶胱硫醚-β-合成酶和胱硫醚-γ-赖氨酸酶的免疫组化分析。数据以阳性组织染色的百分比表示,组间差异采用双向方差分析进行检验。结果显示,催产素和 H2S 系统在组间无明显差异;但性别对催产素系统有影响,ELS 对催产素和 H2S 系统的影响具有性别特异性。没有发现男性对照组 "血清脑损伤标志物 "升高与免疫组化相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Sex and Early Life Stress Experience on Porcine Cardiac and Brain Tissue Expression of the Oxytocin and H2S Systems.

Early life stress (ELS) significantly increases the risk of chronic cardiovascular diseases and may cause neuroinflammation. This post hoc study, based on the material available from a previous study showing elevated "serum brain injury markers" in male control animals, examines the effect of sex and/or ELS on the cerebral and cardiac expression of the H2S and oxytocin systems. Following approval by the Regional Council of Tübingen, a randomized controlled study was conducted on 12 sexually mature, uncastrated German Large White swine of both sexes. The control animals were separated from their mothers at 28-35 days, while the ELS group was separated at day 21. At 20-24 weeks, animals underwent anesthesia, ventilation, and surgical instrumentation. An immunohistochemical analysis of oxytocin, its receptor, and the H2S-producing enzymes cystathionine-β-synthase and cystathionine-γ-lyase was performed on hypothalamic, prefrontal cortex, and myocardial tissue samples. Data are expressed as the % of positive tissue staining, and differences between groups were tested using a two-way ANOVA. The results showed no significant differences in the oxytocin and H2S systems between groups; however, sex influenced the oxytocin system, and ELS affected the oxytocin and H2S systems in a sex-specific manner. No immunohistochemical correlate to the elevated "serum brain injury markers" in male controls was identified.

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来源期刊
Biomolecules
Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
9.40
自引率
3.60%
发文量
1640
审稿时长
18.28 days
期刊介绍: Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications.  Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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