来自人类牙周韧带干细胞的外泌体促进成骨细胞样细胞的分化和大鼠髑髅骨的骨愈合

IF 4.8 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Biomolecules Pub Date : 2024-11-17 DOI:10.3390/biom14111455
Mhd Safwan Albougha, Hideki Sugii, Orie Adachi, Bara Mardini, Serina Soeno, Sayuri Hamano, Daigaku Hasegawa, Shinichiro Yoshida, Tomohiro Itoyama, Junko Obata, Hidefumi Maeda
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引用次数: 0

摘要

深龋和严重牙周炎会导致牙周组织骨吸收,严重的骨吸收会导致牙齿脱落。牙周韧带干细胞(PDLSCs)对缺损牙周组织的愈合非常重要。越来越多的人认识到,牙周组织的愈合是通过分泌营养因子,特别是外泌体介导的。本研究调查了人PDLSCs外泌体(HPDLSCs-Exo)对体外人成骨细胞样细胞和体内大鼠犊骨缺损愈合的影响。HPDLSCs-Exo 被分离出来,并根据其颗粒形状、大小(133 ± 6.4 nm)和表面标志物(CD9、CD63 和 CD81)的表达进行了表征。体外实验结果表明,HPDLSCs-Exo能促进人骨母细胞样细胞的迁移、矿化和骨相关基因的表达,如碱性磷酸酶(ALP)、骨形态发生蛋白2(BMP2)、骨钙素(OCN)和骨生成素(OPN)。此外,体内实验结果表明,在大鼠的缺损部位,HPDLSCs-Exo 处理组比未处理组观察到更多新形成的骨。这些结果表明,HPDLSCs-Exo 在体外和体内都能促进骨生成,这表明 HPDLSCs-Exo 可能是治疗牙周组织缺损骨愈合的一种有吸引力的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exosomes from Human Periodontal Ligament Stem Cells Promote Differentiation of Osteoblast-like Cells and Bone Healing in Rat Calvarial Bone.

Deep caries and severe periodontitis cause bone resorption in periodontal tissue, and severe bone resorption leads to tooth loss. Periodontal ligament stem cells (PDLSCs) are important for the healing of defective periodontal tissue. It is increasingly understood that healing of periodontal tissue is mediated through the secretion of trophic factors, particularly exosomes. This study investigated the effects of exosomes from human PDLSCs (HPDLSCs-Exo) on human osteoblast-like cells in vitro and on the healing of rat calvarial bone defects in vivo. HPDLSCs-Exo were isolated and characterized by their particle shape, size (133 ± 6.4 nm), and expression of surface markers (CD9, CD63, and CD81). In vitro results showed that HPDLSCs-Exo promoted the migration, mineralization, and expression of bone-related genes such as alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), osteocalcin (OCN), and osteopontin (OPN) in human osteoblast-like cells. Furthermore, in vivo results showed that more newly formed bone was observed in the HPDLSCs-Exo-treated group than in the non-treated group at the defect sites in rats. These results indicated that HPDLSCs-Exo could promote osteogenesis in vitro and in vivo, and this suggests that HPDLSCs-Exo may be an attractive treatment tool for bone healing in defective periodontal tissue.

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来源期刊
Biomolecules
Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
9.40
自引率
3.60%
发文量
1640
审稿时长
18.28 days
期刊介绍: Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications.  Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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