高电荷蛋白质形成生物冷凝物的驱动力：二元复合物形成的热力学分析。

IF 4.8 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biomolecules Pub Date : 2024-11-08 DOI:10.3390/biom14111421

Matthias Ballauff

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引用次数: 0

摘要

本文详细分析了带高正电荷的连接子组蛋白 H1 和带高负电荷的伴侣蛋白 Prothymosin α（ProTα）形成二元复合物的热力学过程。ProTα 和 H1 带有大量相反的净电荷（分别为 -44 和 +53），并在生理盐浓度下以高亲和力形成复合物。所获得的二元复合物形成数据是通过一个基于水合效应调制的反离子凝聚的热力学模型进行分析的。分析表明，主要与 ProTα 结合的反离子的释放是主要驱动力，与水释放有关的效应在误差范围内不起作用。研究发现，由于构象自由度的损失，Δcp（=-0.87 kJ/(K mol)）呈强烈负值。

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Driving Forces in the Formation of Biocondensates of Highly Charged Proteins: A Thermodynamic Analysis of the Binary Complex Formation.

A thermodynamic analysis of the binary complex formation of the highly positively charged linker histone H1 and the highly negatively charged chaperone prothymosin α (ProTα) is detailed. ProTα and H1 have large opposite net charges (-44 and +53, respectively) and form complexes at physiological salt concentrations with high affinities. The data obtained for the binary complex formation are analyzed by a thermodynamic model that is based on counterion condensation modulated by hydration effects. The analysis demonstrates that the release of the counterions mainly bound to ProTα is the main driving force, and effects related to water release play no role within the limits of error. A strongly negative Δc_p (=-0.87 kJ/(K mol)) is found, which is due to the loss of conformational degrees of freedom.

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来源期刊

Biomolecules Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

9.40

自引率

3.60%

发文量

1640

审稿时长

18.28 days

期刊介绍： Biomolecules (ISSN 2218-273X) is an international, peer-reviewed open access journal focusing on biogenic substances and their biological functions, structures, interactions with other molecules, and their microenvironment as well as biological systems. Biomolecules publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.