Melina Campos, Gordana Rašić, João Viegas, Anthony J. Cornel, João Pinto, Gregory C. Lanzaro
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引用次数: 0
摘要
疟疾病媒按蚊广泛分布于西非,是圣多美和普林西比群岛上唯一的病媒物种。我们之所以对这些岛屿上这一物种的种群遗传学感兴趣,是为了评估这些岛屿是否适合进行释放基因工程疟蚊的实地试验。该工程构建体包括两个编码抗疟原虫肽的基因,以及一个基于 Cas9 的基因驱动。我们调查了每个岛屿上 A. coluzzii 亚群之间的基因流动情况,以估计不同地点之间的扩散率。取样范围覆盖了两座岛屿上 A. coluzzii 的已知分布区。空间自相关性表明,该物种的扩散范围可能为 7 公里,而基于卷积神经网络的估计值约为 3 公里。这一差异凸显了扩散动态的复杂性和使用多种方法的价值。我们的分析还显示,每个岛屿上的种群之间存在微弱的异质性,但也确定了基因流动的微弱阻力区或容许区。总体而言,两个岛上的 A. coluzzii 都是单一的孟德尔种群。我们预计,包含低阈值基因驱动且对适应性影响最小的基因构建体一旦被引入,就会相对不受阻碍地在每个岛上传播。
Patterns of Gene Flow in Anopheles coluzzii Populations From Two African Oceanic Islands
The malaria vector Anopheles coluzzii is widespread across West Africa and is the sole vector species on the islands of São Tomé and Príncipe. Our interest in the population genetics of this species on these islands is part of an assessment of their suitability for a field trial involving the release of genetically engineered A. coluzzii. The engineered construct includes two genes that encode anti-Plasmodium peptides, along with a Cas9-based gene drive. We investigated gene flow among A. coluzzii subpopulations on each island to estimate dispersal rates between sites. Sampling covered the known range of A. coluzzii on both islands. Spatial autocorrelation suggests 7 km to be the likely extent of dispersal of this species, whereas estimates based on a convolutional neural network were roughly 3 km. This difference highlights the complexity of dispersal dynamics and the value of using multiple approaches. Our analysis also revealed weak heterogeneity among populations within each island but did identify areas weakly resistant or permissive of gene flow. Overall, A. coluzzii on each of the two islands exist as single Mendelian populations. We expect that a gene construct that includes a low-threshold gene drive and has minimal fitness impact should, once introduced, spread relatively unimpeded across each island.
期刊介绍:
Evolutionary Applications is a fully peer reviewed open access journal. It publishes papers that utilize concepts from evolutionary biology to address biological questions of health, social and economic relevance. Papers are expected to employ evolutionary concepts or methods to make contributions to areas such as (but not limited to): medicine, agriculture, forestry, exploitation and management (fisheries and wildlife), aquaculture, conservation biology, environmental sciences (including climate change and invasion biology), microbiology, and toxicology. All taxonomic groups are covered from microbes, fungi, plants and animals. In order to better serve the community, we also now strongly encourage submissions of papers making use of modern molecular and genetic methods (population and functional genomics, transcriptomics, proteomics, epigenetics, quantitative genetics, association and linkage mapping) to address important questions in any of these disciplines and in an applied evolutionary framework. Theoretical, empirical, synthesis or perspective papers are welcome.