从一名携带 STK11 基因突变的 Peutz-Jeghers 综合征患者身上获得 TSHSUi002-A 诱导多能干细胞系

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-11-22 DOI:10.1016/j.scr.2024.103618

Weibo Chen , Hongjuan Wang , Kongxi Zhu , Teng Wang , Weihua Yu , Qiong Wu

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引用次数: 0

摘要

我们报告了从一名携带STK11基因杂合c.909C>G突变的Peutz-Jeghers综合征患者身上获得的诱导多能干细胞（iPSC）系，命名为TSHSUi002-A。这些 iPSCs 是通过使用表达 OCT4、SOX2、KLF4、BCL-XL 和 c-MYC 的外显子载体的非整合方法对外周血单核细胞进行重编程生成的。这些 iPSCs 具有多能性标记，能够在体外分化成所有三个生殖层的细胞，并保持正常的核型。

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Generation of the TSHSUi002-A induced pluripotent stem cell line from a patient with Peutz-Jeghers syndrome carring STK11 gene mutation

We report the derivation of an induced pluripotent stem cell (iPSC) line, designated TSHSUi002-A, from a patient with Peutz-Jeghers syndrome carrying a heterozygous c.909C>G mutation in the STK11 gene. The iPSCs were generated through the reprogramming of peripheral blood mononuclear cells using a non-integrating method involving episomal vectors expressing OCT4, SOX2, KLF4, BCL-XL, and c-MYC. These iPSCs exhibit pluripotency markers, are capable of differentiating into cells of all three germ layers in vitro, and maintain a normal karyotype.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.