基因组尺度代谢模型准确预测佐芬吉色蝇的葡萄糖发酵过程

IF 4.6 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Michelle Meagher , Alex Metcalf , Mark Vigliotti , S. Alex Ramsey , Walter Prentice , Luca Cohen , Shivani Upadhyaya , Melissa S. Roth , Nanette R. Boyle
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引用次数: 0

摘要

藻类有可能成为可再生燃料和化学品的来源。其中一株特殊的藻类,Chromochloris zofingiensis,由于同时生产三酰甘油(TAGs)和虾青素(一种有价值的营养保健品)而备受关注。为了帮助未来的工程设计工作,我们开发了第一个 C. zofingiensis 基因组规模的代谢模型 iCzof1915。该模型包括 1915 个基因、3449 个代谢反应和 2682 种代谢物,横跨 9 个细胞区。我们对自养、混养和异养三种生长条件进行了详细的生物量组成分析,并利用这些数据为每种生长条件制定了生物量形成方程。完成的模型随后用于预测每种生长条件下的通量分布;有趣的是,对于异养生长,该模型预测了发酵产物因溢出代谢而排出体外的情况。我们通过废培养基和发酵产物测定的代谢组学实验证实了这一点。我们还对该模型进行了基因本质分析,以评估在各种生长条件下代谢的稳健性。在这项工作中,我们首次提出了 C. zofingiensis 的基因组尺度代谢模型,并证明了该模型可用于预测不同生长条件下的代谢活动,为该生物体未来的代谢工程研究奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-scale metabolic model accurately predicts fermentation of glucose by Chromochloris zofingiensis
Algae have the potential to be sources of renewable fuels and chemicals. One particular strain, Chromochloris zofingiensis, is of interest due to the co-production of triacylglycerols (TAGs) and astaxanthin, a valuable nutraceutical. To aid in future engineering efforts, we have developed the first genome-scale metabolic model on C. zofingiensis, iCzof1915. This model includes 1915 genes, 3449 metabolic reactions, and 2682 metabolites across 9 cellular compartments. We performed detailed biomass composition analysis for three growth conditions: autotrophic, mixotrophic and heterotrophic and used this data to develop biomass formation equations for each growth condition. The completed model was then used to predict flux distributions for each growth condition; interestingly, for heterotrophic growth, the model predicts the excretion of fermentation products due to overflow metabolism. We confirmed this experimentally via metabolomics of spent medium and fermentation product assays. An in silico gene essentiality analysis was also performed on this model to evaluate metabolism robustness in each growth condition. In this work, we present the first genome-scale metabolic model of C. zofingiensis and demonstrate its use predicting metabolic activity in different growth conditions, setting up a foundation for future metabolic engineering studies in this organism.
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来源期刊
Algal Research-Biomass Biofuels and Bioproducts
Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
9.40
自引率
7.80%
发文量
332
期刊介绍: Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment
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