促进创伤感染的耐多药金黄色葡萄球菌小鼠伤口愈合的原薯蓣皂苷水凝胶

IF 5.2 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Xin Wang, Guangfan Meng, Zongyu Zhang, Jiacheng Zhao, Shaoyu Wang, Dongliang Hua, JingZhang, Jie Zhang
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引用次数: 0

摘要

长期以来,耐多药金黄色葡萄球菌(MRSA)引起的伤口感染一直被视为临床上的难题。本研究基于原肌苷(PG)卓越的抗菌效果和水凝胶敷料在组织修复和再生方面的能力,制备了 PG 水凝胶,用于治疗 MRSA 引起的伤口感染。流变学测试表明,PG 水凝胶作为一种半固体凝胶具有良好的机械性能。体外药物渗透研究和皮肤动力学研究表明,PG 水凝胶具有较高的 PG 渗透性,并能在皮肤中短期滞留。此外,对小鼠皮肤伤口的体内实验表明,使用 PG 水凝胶处理后,小鼠血清中的 IL-β 等炎症因子水平降低,组织的炎症浸润减少,COL1A1 等基因的转录水平在伤口愈合的不同阶段上调,脱硫弧菌等菌属的相对丰度降低,从而促进了小鼠伤口的愈合。我们的研究将为临床上治疗 MRSA 感染的药物短缺提供新的解决方案,并为提高 PG 的综合价值提供研究基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Prodigiosin hydrogel to promote healing of trauma-infected multidrug-resistant Staphylococcus aureus mice wounds

Prodigiosin hydrogel to promote healing of trauma-infected multidrug-resistant Staphylococcus aureus mice wounds
Wound infections caused by Multidrug-resistant Staphylococcus aureus (MRSA) have been regarded as a challenging problem in clinic for the long time. In this study, based on the excellent antimicrobial effect of prodigiosin(PG) and the ability of hydrogel dressing in terms of tissue repair and regeneration, we prepared the PG hydrogel as a treatment for the wound infection induced by MRSA. Rheological tests indicated that PG hydrogel as a semi-solid gel had good mechanical properties. In ex vitro drug permeation studies and dermatokinetic studies showed that PG hydrogel had high PG permeability and were capable of short-term retention in the skin. In addition, in vivo experiments for mouse skin wounds showed that the serum levels of inflammatory factors including IL-β and other inflammatory factors were reduced, the inflammatory infiltration of tissues was reduced, the transcript levels of genes such as COL1A1 were up-regulated at different stages of wound healing, and the relative abundance of genera such as Desulfovibrio was lowered after treatment with PG hydrogel, which facilitated wound healing in mice. Our study would provide a new solution to the clinical shortage of drugs for the treatment of MRSA infection and provide a research basis for improving the comprehensive values of PG.
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来源期刊
International Journal of Pharmaceutics: X
International Journal of Pharmaceutics: X Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
6.60
自引率
0.00%
发文量
32
审稿时长
24 days
期刊介绍: International Journal of Pharmaceutics: X offers authors with high-quality research who want to publish in a gold open access journal the opportunity to make their work immediately, permanently, and freely accessible. International Journal of Pharmaceutics: X authors will pay an article publishing charge (APC), have a choice of license options, and retain copyright. Please check the APC here. The journal is indexed in SCOPUS, PUBMED, PMC and DOAJ. The International Journal of Pharmaceutics is the second most cited journal in the "Pharmacy & Pharmacology" category out of 358 journals, being the true home for pharmaceutical scientists concerned with the physical, chemical and biological properties of devices and delivery systems for drugs, vaccines and biologicals, including their design, manufacture and evaluation. This includes evaluation of the properties of drugs, excipients such as surfactants and polymers and novel materials. The journal has special sections on pharmaceutical nanotechnology and personalized medicines, and publishes research papers, reviews, commentaries and letters to the editor as well as special issues.
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