山羊奶中富含的α2,6-连接的糖基化低聚糖通过调节肠道菌群和粘蛋白 O-糖基化缓解食物过敏症

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED
Cheng Li , Tianjiao Han, Peiyun Zhong, Yuyang Zhang, Tong Zhao, Shukai Wang, Xiaoqin Wang, Yang Tian, Guiping Gong, Yuxia Liu, Linjuan Huang, Yu Lu, Zhongfu Wang
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引用次数: 0

摘要

营养丰富的羊奶过敏性低。低聚糖是羊奶中重要的功能性成分之一,其结构与人奶低聚糖(HMOs)相似。目前,还没有关于转基因生物抗过敏活性的报道。在这项研究中,GMOs 被有效地分离成中性(NGMOs)和硅烷基化(SGMOs)组分,然后使用在线 LC-MS/MS 进行异构体水平的定性和定量分析。在山羊奶中检测到 15 种 NGMOs 和 28 种 SGMOs,其中 10 种 SGMOs 是首次报道。其中,α2,6-连接的 SGMOs 在山羊奶中的含量是牛乳的 3.9 倍,SGMOs 中 6′SL、3′SLN 和 6′NGL的总相对含量接近 60%,与 HMOs 更为相似。口服转基因生物,尤其是α2,6-连接的硅烷基化寡糖,可明显缓解卵清蛋白诱导的 BALB/c 小鼠的食物过敏症状。SGMOs恢复了Lachnospiraceae、Erysipelotrichaceae和Bacteroidaceae的平衡,重建了肠道粘膜屏障,特别是恢复了粘蛋白O型糖的岩藻糖基化、硅烷基化和硫酸化水平,显著提高了四种核心2型O型糖(F1H2N2、F2H2N2、S1F2H2N2和A1F1H2N2)的表达。这是首次对转基因生物抗过敏活性的全面研究,研究结果为开发基于转基因生物的天然抗过敏成分奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

α2,6-linked sialylated oligosaccharides riched in goat milk alleviate food allergy by regulating the gut flora and mucin O-glycosylation

α2,6-linked sialylated oligosaccharides riched in goat milk alleviate food allergy by regulating the gut flora and mucin O-glycosylation
The nutritious goat milk has low allergenicity. Oligosaccharides represent one of the crucial functional constituents in goat milk, which are structurally similar to human milk oligosaccharides (HMOs). Currently, the anti-allergic activity of GMOs has not been reported. In this study, GMOs were efficiently separated into neutral (NGMOs) and sialylated (SGMOs) fractions, following by qualitative and quantitative analysis at the isomer level using online LC-MS/MS. Fifteen NGMOs and 28 SGMOs were detected in goat milk, with 10 SGMOs reported for the first time. Distinctly, α2,6-linked SGMOs were 3.9 times more abundant in goat milk than in bovine milk, with the total relative content of 6′SL, 3′SLN and 6′NGL in SGMOs approach to 60%, which is more similar to HMOs. Orally administering GMOs, especially α2,6-linked sialylated oligosaccharides, significantly alleviated food allergy in ovalbumin-induced BALB/c mice. SGMOs restored the balance of Lachnospiraceae, Erysipelotrichaceae, and Bacteroidaceae, reconstructed the intestinal mucosal barrier, especially restored the levels of fucosylation, sialylation, and sulfation of mucin O-glycans, increased the expression of four core type 2 O-glycans (F1H2N2, F2H2N2, S1F2H2N2, and A1F1H2N2) significantly. This is the first comprehensive study of the anti-allergic activity of GMOs, and the results lay the foundation for the development of GMOs-based natural anti-allergic components.
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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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