Xenorhabdus bharatensis sp.nov.、Xenorhabdus entomophaga sp.nov.、Xenorhabdus siamensis sp.nov.和 Xenorhabdus thailandensis sp.nov.从 Steinernema Entomopathogenic 线虫中分离出来。

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-11-25 DOI:10.1007/s00284-024-03972-7

Aunchalee Thanwisai, Ricardo A R Machado, Aashaq Hussain Bhat, Sacha J Pidot, Sarunporn Tandhavanant, Chanakan Subkrasae, Wipanee Meesil, Jiranun Ardpairin, Supawan Pansri, Apichat Vitta

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引用次数: 0

摘要

研究发现，从 Steinernema 昆虫病原线虫中分离出的四株革兰氏染色阴性细菌 CS20T、AUT15.5T、XENO-11T 和 CCN3.3T 代表了 Xenorhabdus 属（Gammaproteobacteria，摩根菌科）中的新物种。在这项研究中，我们利用全基因组系统发生组重建、核心基因组序列的序列同一性值和表型特征描述了这些新物种。基于 16S rRNA 基因序列的系统进化重建表明(i) 菌株 CS20T 与 X. stockiae DSM 17904T 关系密切；(ii) 菌株 AUT15.5T 与 X. budapestensis DSM 16342T 关系密切；(iii) 菌株 XENO-11T 与 X. khoisanae DSM 25463T 关系密切；(iv) 菌株 CCN3.3T 与 X. griffiniae DSM 17911T 关系密切。菌株 CS20T 与 X. stockiae DSM 17904T 的 16S rRNA 基因序列相似度为 97.8%，菌株 AUT15.5T 与 X. budapestensis DSM 16342T 的 16S rRNA 基因序列相似度为 98.1%，菌株 XENO-11T 与 X. khoisanae DSM 25463T 的 16S rRNA 基因序列相似度为 97.8%，菌株 CCN3.3T 与 X. griffiniae DSM 17911T 的 16S rRNA 基因序列相似度为 98.6%。利用全基因组序列进行的系统发生组重建表明(i) 菌株 CS20T 与 X. stockiae DSM 17904T 和 X. innexi DSM 16336T 关系密切；(ii) 菌株 AUT15.5T 与 X. indica DSM 17382T 关系密切；(iii) 菌株 XENO-11T 与 X. khoisanae DSM 25463T 关系密切；(iv) 菌株 CCN3.3T 与 X. griffiniae DSM 17911T 关系密切。CS20T、AUT15.5T、XENO-11T和CCN3.3T菌株的数字DNA-DNA杂交（dDDH）值和平均核苷酸同一性（ANI）值分别低于原核生物物种划分所用的70%和95-96%的差异临界值。因此，我们提出以下四个新物种：Xenorhabdus bharatensis sp.siamensis sp. nov.（模式菌株为 AUT15.5T=CCM 9405T=CCOS 2116T），以及 X. thailandensis sp. nov.（模式菌株为 CCN3.3T=CCM 9406T=CCOS 2115T）。以下生化测试可能有助于区分这些新物种和与其关系密切的类群：乙酰辅酶生成、精氨酸二水解酶、柠檬酸利用、明胶酶、葡萄糖氧化、吲哚生成和色氨酸脱氨酶。我们的研究有助于更好地了解与昆虫寄生线虫相关的昆虫病原菌的生物多样性和系统发育关系。

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Xenorhabdus bharatensis sp. nov., Xenorhabdus entomophaga sp. nov., Xenorhabdus siamensis sp. nov., and Xenorhabdus thailandensis sp. nov. Isolated from Steinernema Entomopathogenic Nematodes.

Four Gram-stain-negative bacterial strains, CS20^T, AUT15.5^T, XENO-11^T, and CCN3.3^T, isolated from Steinernema entomopathogenic nematodes, were found to represent novel species within the genus Xenorhabdus (Gammaproteobacteria, Morganellaceae). In this study, we described these new species using whole-genome phylogenomic reconstructions, sequence identity values from core genome sequences, and phenotypic characterization. Phylogenetic reconstructions based on 16S rRNA gene sequences showed that: (i) strain CS20^T is closely related to X. stockiae DSM 17904^T, (ii) strain AUT15.5^T is closely related to X. budapestensis DSM 16342^T, (iii) strain XENO-11^T is closely related to X. khoisanae DSM 25463^T, and (iv) strain CCN3.3^T is closely related to X. griffiniae DSM 17911^T. The 16S rRNA gene sequence similarity value between strain CS20^T and X. stockiae DSM 17904^T is 97.8%, between strain AUT15.5^T and X. budapestensis DSM 16342^T is 98.1%, between strain XENO-11^T and X. khoisanae DSM 25463^T is 97.8%, and between strain CCN3.3^T and X. griffiniae DSM 17911^T is 98.6%. Phylogenomic reconstructions using whole-genome sequences showed that: (i) strain CS20^T is closely related to X. stockiae DSM 17904^T and X. innexi DSM 16336^T, (ii) strain AUT15.5^T is closely related to X. indica DSM 17382^T, (iii) strain XENO-11^T is closely related to X. khoisanae DSM 25463^T, and (iv) strain CCN3.3^T is closely related to X. griffiniae DSM 17911^T. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains CS20^T, AUT15.5^T, XENO-11^T, and CCN3.3^T and the type strains of their more closely related species are below the 70% and the 95-96% divergence thresholds, respectively, used for prokaryotic species delineation. Hence, we propose the following four new species: Xenorhabdus bharatensis sp. nov. (the type strain is CS20^T=CCM 9320^T=CCOS 2070^T), X. entomophaga sp. nov. (the type strain is XENO-11^T=CCM 9389^T=CCOS 2111^T), X. siamensis sp. nov. (the type strain is AUT15.5^T=CCM 9405^T=CCOS 2116^T), and X. thailandensis sp. nov. (the type strain is CCN3.3^T=CCM 9406^T=CCOS 2115^T). The following biochemical tests may be useful for differentiating the novel species from their more closely related taxa: acetoin production, arginine dihydrolase, citrate utilization, gelatinase, glucose oxidation, indole production, and tryptophan deaminase. Our study contributes to a better understanding of the biodiversity and phylogenetic relationships of entomopathogenic bacteria associated with insect parasitic nematodes.

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.