{"title":"皮肤黑色素瘤的新型预后标志物","authors":"Yi Zhang, Ansheng Xie, Di Wang, Weiwei Deng","doi":"10.2147/CCID.S486679","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Skin cutaneous melanoma (SKCM) ranks among the most prevalent malignant tumors, highlighting the significance of identifying new research targets. In this study, our objective was to pinpoint pivotal genes implicated in SKCM pathogenesis and ascertain their potential as prognostic biomarkers.</p><p><strong>Methods: </strong>Leveraging data from 1809 normal skin samples and 471 SKCM samples, we identified differentially expressed genes (DEGs). Using a comprehensive suite of bioinformatic analyses, including weighted gene co-expression network analysis (WGCNA), we elucidated the functions of these DEGs and singled out hub genes. Cox analyses and overall survival analyses underscored that elevated expression of these genes correlated with more favorable prognoses.</p><p><strong>Results: </strong>Ultimately, we identified five genes (<i>PLAC8, IL4I1, ZNF80, CCR8, CLEC4C</i>) as novel prognostic markers for SKCM. Furthermore, multivariate Cox analyses pinpointed <i>ZNF80</i> and <i>CCR8</i> as independent prognostic biomarkers. Experimental validation targeting these genes revealed significant downregulation in melanoma cells, except for <i>CCR8</i>. Subsequent knockdown of <i>IL4I1</i> promoted both the proliferation and inhibited the apoptosis of melanoma cells.</p><p><strong>Conclusion: </strong>In summary, our study identified a series of potential prognostic genes in melanoma and verified the functional role of <i>IL4I1</i> among them.</p>","PeriodicalId":10447,"journal":{"name":"Clinical, Cosmetic and Investigational Dermatology","volume":"17 ","pages":"2615-2625"},"PeriodicalIF":1.9000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586483/pdf/","citationCount":"0","resultStr":"{\"title\":\"Novel Prognostic Markers for Skin Cutaneous Melanoma.\",\"authors\":\"Yi Zhang, Ansheng Xie, Di Wang, Weiwei Deng\",\"doi\":\"10.2147/CCID.S486679\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Skin cutaneous melanoma (SKCM) ranks among the most prevalent malignant tumors, highlighting the significance of identifying new research targets. In this study, our objective was to pinpoint pivotal genes implicated in SKCM pathogenesis and ascertain their potential as prognostic biomarkers.</p><p><strong>Methods: </strong>Leveraging data from 1809 normal skin samples and 471 SKCM samples, we identified differentially expressed genes (DEGs). Using a comprehensive suite of bioinformatic analyses, including weighted gene co-expression network analysis (WGCNA), we elucidated the functions of these DEGs and singled out hub genes. Cox analyses and overall survival analyses underscored that elevated expression of these genes correlated with more favorable prognoses.</p><p><strong>Results: </strong>Ultimately, we identified five genes (<i>PLAC8, IL4I1, ZNF80, CCR8, CLEC4C</i>) as novel prognostic markers for SKCM. Furthermore, multivariate Cox analyses pinpointed <i>ZNF80</i> and <i>CCR8</i> as independent prognostic biomarkers. Experimental validation targeting these genes revealed significant downregulation in melanoma cells, except for <i>CCR8</i>. Subsequent knockdown of <i>IL4I1</i> promoted both the proliferation and inhibited the apoptosis of melanoma cells.</p><p><strong>Conclusion: </strong>In summary, our study identified a series of potential prognostic genes in melanoma and verified the functional role of <i>IL4I1</i> among them.</p>\",\"PeriodicalId\":10447,\"journal\":{\"name\":\"Clinical, Cosmetic and Investigational Dermatology\",\"volume\":\"17 \",\"pages\":\"2615-2625\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586483/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical, Cosmetic and Investigational Dermatology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2147/CCID.S486679\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical, Cosmetic and Investigational Dermatology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/CCID.S486679","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"DERMATOLOGY","Score":null,"Total":0}
Novel Prognostic Markers for Skin Cutaneous Melanoma.
Background: Skin cutaneous melanoma (SKCM) ranks among the most prevalent malignant tumors, highlighting the significance of identifying new research targets. In this study, our objective was to pinpoint pivotal genes implicated in SKCM pathogenesis and ascertain their potential as prognostic biomarkers.
Methods: Leveraging data from 1809 normal skin samples and 471 SKCM samples, we identified differentially expressed genes (DEGs). Using a comprehensive suite of bioinformatic analyses, including weighted gene co-expression network analysis (WGCNA), we elucidated the functions of these DEGs and singled out hub genes. Cox analyses and overall survival analyses underscored that elevated expression of these genes correlated with more favorable prognoses.
Results: Ultimately, we identified five genes (PLAC8, IL4I1, ZNF80, CCR8, CLEC4C) as novel prognostic markers for SKCM. Furthermore, multivariate Cox analyses pinpointed ZNF80 and CCR8 as independent prognostic biomarkers. Experimental validation targeting these genes revealed significant downregulation in melanoma cells, except for CCR8. Subsequent knockdown of IL4I1 promoted both the proliferation and inhibited the apoptosis of melanoma cells.
Conclusion: In summary, our study identified a series of potential prognostic genes in melanoma and verified the functional role of IL4I1 among them.
期刊介绍:
Clinical, Cosmetic and Investigational Dermatology is an international, peer-reviewed, open access journal that focuses on the latest clinical and experimental research in all aspects of skin disease and cosmetic interventions. Normal and pathological processes in skin development and aging, their modification and treatment, as well as basic research into histology of dermal and dermal structures that provide clinical insights and potential treatment options are key topics for the journal.
Patient satisfaction, preference, quality of life, compliance, persistence and their role in developing new management options to optimize outcomes for target conditions constitute major areas of interest.
The journal is characterized by the rapid reporting of clinical studies, reviews and original research in skin research and skin care.
All areas of dermatology will be covered; contributions will be welcomed from all clinicians and basic science researchers globally.