Shikun Hou, Nathaly M Arce Soto, Elizabeth J Glover
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However, the desire to examine drinking patterns in the context of overall intake, pharmacological interventions, and neuronal manipulations has prompted the development of home cage lick detection systems that can acquire data at the level of individual licks. Although a number of these systems have been developed recently, the open-source system, LIQ HD (Lick Instance Quantifier Home cage Device), has garnered significant attention in the field for its affordability and user friendliness. Although exciting, this system was designed for use in mice. Here, we review appropriate procedures for standard and lickometer-equipped two-bottle choice home cage drinking. We also introduce methods for adapting the LIQ HD system to rats including hardware modifications to accommodate larger cage size and a redesigned 3D printed bottle holder compatible with standard off-the-shelf drinking bottles. 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引用次数: 0
摘要
双瓶选择家庭笼饮是研究啮齿类动物乙醇消耗最广泛使用的范例之一。最简单的方法是让动物使用两个饮水瓶,其中一个装有普通自来水,另一个装有乙醇,每天的摄入量通过24小时内饮水瓶重量的变化来测量。因此,这种方法不需要专门的实验室设备。虽然这种方法易于实施,可能是临床前酒精研究人员广泛采用这种方法的最大原因,但使用这种方法获得的饮酒数据的分辨率受到研究人员测量瓶子重量次数的限制(例如,每天一次)。然而,由于希望结合总体摄入量、药物干预和神经元操作来研究饮酒模式,人们开发了家庭笼舔检测系统,该系统可获取单次舔舐的数据。尽管最近已经开发出了许多这样的系统,但开源系统 LIQ HD(舔舐实例定量家庭笼设备)因其经济实惠和用户友好性而在该领域备受关注。尽管令人兴奋,但该系统是专为小鼠设计的。在此,我们回顾了标准和配备舔量计的双瓶选择家庭笼饮酒的适当程序。我们还介绍了使 LIQ HD 系统适用于大鼠的方法,包括修改硬件以适应更大的笼子尺寸,以及重新设计与标准现成饮水瓶兼容的 3D 打印瓶架。利用这种方法,研究人员可以同时检测多只大鼠的日常饮水模式和摄入水平,从而以最小的额外资源投资提高所获数据的分辨率。这些方法为研究人员提供了灵活性,他们可以根据自己的精确实验需求,使用标准酒瓶或配备舔测仪的仪器来研究饮酒的神经生物学机制。
Modeling Alcohol Consumption in Rodents Using Two-Bottle Choice Home Cage Drinking and Microstructural Analysis.
Two-bottle choice home cage drinking is one of the most widely used paradigms to study ethanol consumption in rodents. In its simplest form, animals are provided with access to two drinking bottles, one of which contains regular tap water and the other ethanol, with daily intake measured by change in bottle weight over the 24 h period. Consequently, this approach requires no specialized laboratory equipment. While such ease of implementation is likely the greatest contributor to its widespread adoption by preclinical alcohol researchers, the resolution of drinking data acquired using this approach is limited by the number of times the researcher measures bottle weight (e.g., once daily). However, the desire to examine drinking patterns in the context of overall intake, pharmacological interventions, and neuronal manipulations has prompted the development of home cage lick detection systems that can acquire data at the level of individual licks. Although a number of these systems have been developed recently, the open-source system, LIQ HD (Lick Instance Quantifier Home cage Device), has garnered significant attention in the field for its affordability and user friendliness. Although exciting, this system was designed for use in mice. Here, we review appropriate procedures for standard and lickometer-equipped two-bottle choice home cage drinking. We also introduce methods for adapting the LIQ HD system to rats including hardware modifications to accommodate larger cage size and a redesigned 3D printed bottle holder compatible with standard off-the-shelf drinking bottles. Using this approach, researchers can examine daily drinking patterns in addition to levels of intake in many rats in parallel thereby increasing the resolution of acquired data with minimal investment in additional resources. These methods provide researchers with the flexibility to use either standard bottles or a lickometer-equipped apparatus to interrogate the neurobiological mechanisms underlying alcohol drinking depending on their precise experimental needs.
期刊介绍:
JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.