定量化成年果蝇外部结构的快速、简单工作流程

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Laura Martins, Ramy Wong, Fredrick J Larabee, Bree K Grillo-Hill
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引用次数: 0

摘要

果蝇的复眼是一种精确模式化的组织,它揭示了驱动形态发生的分子机制和生物过程。它是一种简单的重复单元眼结构,被称为omatidia,用于描述遗传相互作用和基因功能。影响眼球结构的突变很容易检测和分析,因此,资源不足的研究机构经常使用这一系统。进一步的表型分析通常包括扫描电子显微镜(SEM),以生成适合定量分析的高倍率图像。然而,扫描电子显微镜价格昂贵,需要昂贵的试剂;样品制备需要数天时间;通常还需要全职工作人员进行样品制备和仪器维护。这就限制了它们在资源不足或预算紧缩的机构中的应用。在昆虫学领域,使用高分辨率数字成像技术是鉴定和描述物种的常用方法。本文介绍了一种方法,该方法结合了多种策略,允许对成年果蝇的结构进行高分辨率数字成像,并使用开放软件 ImageJ 进行定量分析。该工作流程是一种快速且方便学生使用的替代方法,它弥补了资金和资源不足的研究设施的局限性,是一种具有成本效益且快速的表型定量分析方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Rapid, Simple Workflow for Quantification of External Adult Drosophila Structures.

The Drosophila compound eye is a precisely patterned tissue that has revealed molecular mechanisms and biological processes that drive morphogenesis. It is a simple structure of repeating unit eyes, termed ommatidia, that is used to characterize genetic interactions and gene functions. Mutations that affect eye architecture can be easily detected and analyzed; hence, this system is frequently used in under-resourced institutions. Further phenotypic analysis often includes a Scanning Electron Microscope (SEM) to generate high-magnification images suitable for quantitative analysis. However, SEMs are expensive and require costly reagents; sample preparation spans days; and, often, they need full-time staff for sample preparation and instrument maintenance. This limits their utility at under-resourced institutions or during budgetary austerity. In entomology, the use of high-resolution digital imaging technology is a common practice for the identification and characterization of species. This paper describes a method that combines strategies and allows for high-resolution digital imaging of adult Drosophila structures and quantitative analysis using the open software ImageJ. The workflow is a rapid and student-friendly alternative that remedies the limitations of underfunded and under-resourced research facilities with a cost-effective and rapid approach to quantitative phenotypic analysis.

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来源期刊
Jove-Journal of Visualized Experiments
Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-
CiteScore
2.10
自引率
0.00%
发文量
992
期刊介绍: JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.
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