Adriana Aparecida Silva da Costa, Sicília Rezende Oliveira, Thalita Soares Tavares, Daniela Pereira Meirelles, Evânio Vilela da Silva, Anderson Tangerino Ferreira da Silva, Jorge Esquiche León, Sérgio Vitorino Cardoso, Maria Cássia Ferreira de Aguiar, Tarcília Aparecida da Silva, Patrícia Carlos Caldeira
{"title":"人乳头瘤病毒状态对口咽癌中性粒细胞胞外捕获器释放的影响","authors":"Adriana Aparecida Silva da Costa, Sicília Rezende Oliveira, Thalita Soares Tavares, Daniela Pereira Meirelles, Evânio Vilela da Silva, Anderson Tangerino Ferreira da Silva, Jorge Esquiche León, Sérgio Vitorino Cardoso, Maria Cássia Ferreira de Aguiar, Tarcília Aparecida da Silva, Patrícia Carlos Caldeira","doi":"10.1111/jop.13594","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Oropharyngeal squamous cell carcinoma (OP-SCC) represents a public health problem and human papillomavirus (HPV) is one of the risk factors. Neutrophil extracellular traps (NET) are meshes of DNA strands and granule proteins. NET has been identified in diverse cancers, whether associated with viruses or not. However, there is no information on NET in OP-SCC. We aimed to evaluate the NET release by neutrophils in the OP-SCC microenvironment, stratified by HPV status.</p><p><strong>Methods: </strong>This cross-sectional study analyzed OP-SCC biopsy specimens diagnosed from 1997 to 2021. HPV status was determined by p16 immunohistochemistry and \"in situ\" hybridization. Neutrophils were detected by CD66b immunohistochemistry. Immunofluorescence was used to identify NET by co-localization of myeloperoxidase (MPO) and citrullinated histone H3 (H3Cit). Bivariate statistics, Kaplan-Meier survival analysis, and the log-rank test were performed.</p><p><strong>Results: </strong>HPV-positive and HPV-negative OP-SCC had similar CD66b + neutrophil infiltration (p > 0.05), but the release of NET was significantly increased in HPV-positive compared to HPV-negative OP-SCC samples (p < 0.05). Overall survival was not impacted by NET indexes (p > 0.05).</p><p><strong>Conclusion: </strong>The presence of HPV may stimulate NET release in the OP-SCC microenvironment.</p>","PeriodicalId":16588,"journal":{"name":"Journal of Oral Pathology & Medicine","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Contribution of HPV Status for Neutrophil Extracellular Traps Release in Oropharyngeal Cancer.\",\"authors\":\"Adriana Aparecida Silva da Costa, Sicília Rezende Oliveira, Thalita Soares Tavares, Daniela Pereira Meirelles, Evânio Vilela da Silva, Anderson Tangerino Ferreira da Silva, Jorge Esquiche León, Sérgio Vitorino Cardoso, Maria Cássia Ferreira de Aguiar, Tarcília Aparecida da Silva, Patrícia Carlos Caldeira\",\"doi\":\"10.1111/jop.13594\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Oropharyngeal squamous cell carcinoma (OP-SCC) represents a public health problem and human papillomavirus (HPV) is one of the risk factors. Neutrophil extracellular traps (NET) are meshes of DNA strands and granule proteins. NET has been identified in diverse cancers, whether associated with viruses or not. However, there is no information on NET in OP-SCC. We aimed to evaluate the NET release by neutrophils in the OP-SCC microenvironment, stratified by HPV status.</p><p><strong>Methods: </strong>This cross-sectional study analyzed OP-SCC biopsy specimens diagnosed from 1997 to 2021. HPV status was determined by p16 immunohistochemistry and \\\"in situ\\\" hybridization. Neutrophils were detected by CD66b immunohistochemistry. Immunofluorescence was used to identify NET by co-localization of myeloperoxidase (MPO) and citrullinated histone H3 (H3Cit). Bivariate statistics, Kaplan-Meier survival analysis, and the log-rank test were performed.</p><p><strong>Results: </strong>HPV-positive and HPV-negative OP-SCC had similar CD66b + neutrophil infiltration (p > 0.05), but the release of NET was significantly increased in HPV-positive compared to HPV-negative OP-SCC samples (p < 0.05). Overall survival was not impacted by NET indexes (p > 0.05).</p><p><strong>Conclusion: </strong>The presence of HPV may stimulate NET release in the OP-SCC microenvironment.</p>\",\"PeriodicalId\":16588,\"journal\":{\"name\":\"Journal of Oral Pathology & Medicine\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-11-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral Pathology & Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1111/jop.13594\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Pathology & Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/jop.13594","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
背景：口咽鳞状细胞癌（OP-SCC）是一个公共卫生问题，而人类乳头瘤病毒（HPV）是风险因素之一。中性粒细胞胞外捕获物（NET）是由 DNA 链和颗粒蛋白质组成的网状结构。已在多种癌症中发现了中性粒细胞胞外捕获器，无论是否与病毒有关。然而，目前还没有关于 OP-SCC 中 NET 的信息。我们旨在评估中性粒细胞在 OP-SCC 微环境中释放的 NET，并根据 HPV 状态进行分层：这项横断面研究分析了 1997 年至 2021 年期间诊断的 OP-SCC 活检标本。HPV状态通过p16免疫组化和 "原位 "杂交确定。通过CD66b免疫组化检测中性粒细胞。免疫荧光通过髓过氧化物酶（MPO）和瓜氨酸组蛋白H3（H3Cit）的共定位来识别NET。进行了双变量统计、Kaplan-Meier生存分析和对数秩检验：结果：HPV阳性和HPV阴性的OP-SCC具有相似的CD66b +中性粒细胞浸润（P > 0.05），但HPV阳性的OP-SCC样本与HPV阴性的OP-SCC样本相比，NET的释放量显著增加（P 0.05）：结论：HPV 的存在可能会刺激 OP-SCC 微环境中 NET 的释放。
Contribution of HPV Status for Neutrophil Extracellular Traps Release in Oropharyngeal Cancer.
Background: Oropharyngeal squamous cell carcinoma (OP-SCC) represents a public health problem and human papillomavirus (HPV) is one of the risk factors. Neutrophil extracellular traps (NET) are meshes of DNA strands and granule proteins. NET has been identified in diverse cancers, whether associated with viruses or not. However, there is no information on NET in OP-SCC. We aimed to evaluate the NET release by neutrophils in the OP-SCC microenvironment, stratified by HPV status.
Methods: This cross-sectional study analyzed OP-SCC biopsy specimens diagnosed from 1997 to 2021. HPV status was determined by p16 immunohistochemistry and "in situ" hybridization. Neutrophils were detected by CD66b immunohistochemistry. Immunofluorescence was used to identify NET by co-localization of myeloperoxidase (MPO) and citrullinated histone H3 (H3Cit). Bivariate statistics, Kaplan-Meier survival analysis, and the log-rank test were performed.
Results: HPV-positive and HPV-negative OP-SCC had similar CD66b + neutrophil infiltration (p > 0.05), but the release of NET was significantly increased in HPV-positive compared to HPV-negative OP-SCC samples (p < 0.05). Overall survival was not impacted by NET indexes (p > 0.05).
Conclusion: The presence of HPV may stimulate NET release in the OP-SCC microenvironment.
期刊介绍：
The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.
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