依托咪酯通过抑制 PI3K/AKT 通路抑制食管癌细胞的增殖、迁移、侵袭和糖酵解。

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cytotechnology Pub Date : 2025-02-01 Epub Date: 2024-11-20 DOI:10.1007/s10616-024-00661-y
Xiangchao Zhang, Zhengjun Li, Tao Wang
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引用次数: 0

摘要

食管癌仍然是肿瘤学领域的一项艰巨挑战,其特点是预后不良和治疗方案有限。最近的研究揭示了将现有药物重新用于癌症治疗的潜力。值得注意的是,依托咪酯是一种传统上用于诱导全身麻醉的麻醉剂,它已成为一种有前途的候选药物,在各种肿瘤类型中显示出显著的抗癌特性。本研究旨在探讨依托咪酯对食管癌细胞的影响,重点关注其调节 PI3K/AKT 信号通路和抑制肿瘤增殖的能力。本研究采用体外和体内方法评估依托咪酯对食管癌细胞的影响。体外实验评估了依托咪酯对细胞增殖、迁移、侵袭和糖酵解过程的影响。为了研究依托咪酯对肿瘤生长的治疗潜力,并评估其在生理相关背景下对 PI3K/AKT 信号通路的影响,我们建立了一个体内异种移植小鼠模型。依托咪酯对食管癌细胞的增殖、迁移、侵袭和糖酵解能力有明显的抑制作用。这种对致瘤特性的多方面抑制与 PI3K/AKT 通路的抑制密切相关,PI3K 和 AKT 磷酸化水平的降低就是证明。使用食道癌小鼠模型进行的体内研究证实了这些发现。依托咪酯的施用导致肿瘤体积和质量大幅减少,同时肿瘤组织内的凋亡活性增强,PI3K/AKT通路受到抑制。这项研究表明,依托咪酯可通过抑制 PI3K/AKT 通路有效抑制食管癌的进展。这些令人鼓舞的结果证明,依托咪酯作为一种潜在的食管癌治疗策略值得进一步临床研究:在线版本包含补充材料,可在10.1007/s10616-024-00661-y上获取。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Etomidate suppresses proliferation, migration, invasion, and glycolysis in esophageal cancer cells via PI3K/AKT pathway inhibition.

Esophageal cancer remains a formidable challenge in oncology, characterized by its poor prognosis and limited therapeutic options. Recent investigations have unveiled the potential of repurposing existing drugs for cancer treatment. Notably, etomidate, an anesthetic agent traditionally used for inducing general anesthesia, has emerged as a promising candidate demonstrating significant anticancer properties across various tumor types. The present study aims to investigate the effects of etomidate on esophageal carcinoma cells, with a specific focus on its ability to modulate the PI3K/AKT signaling pathway and inhibit tumor proliferation. This study employed both in vitro and in vivo methodologies to assess the effects of etomidate on esophageal cancer cells. In vitro experiments evaluated the effects of etomidate on cell proliferation, migration, invasion, and glycolytic processes. An in vivo xenograft mouse model was established to investigate the therapeutic potential of etomidate on tumor growth and assess its impact on the PI3K/AKT signaling pathway in a physiologically relevant context. Etomidate demonstrated a significant inhibitory effect on the proliferation, migration, invasion, and glycolytic capacity of esophageal cancer cells. This multifaceted suppression of tumorigenic properties was closely associated with the inhibition of the PI3K/AKT pathway, as evidenced by reduced phosphorylation levels of PI3K and AKT. In vivo studies using a murine model of esophageal cancer corroborated these findings. Etomidate administration resulted in a substantial reduction in tumor volume and mass, accompanied by increased apoptotic activity and the inhibition of the PI3K/AKT pathway within the tumor tissue. This study demonstrates etomidate's potent inhibition of esophageal cancer progression through suppression of the PI3K/AKT pathway. These promising results warrant further clinical investigation of etomidate as a potential therapeutic strategy for esophageal cancer.

Supplementary information: The online version contains supplementary material available at 10.1007/s10616-024-00661-y.

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来源期刊
Cytotechnology
Cytotechnology 生物-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
49
审稿时长
6-12 weeks
期刊介绍: The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.
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