Sulfoquinovosyl diacylglycerol 是圣罗勒 Ocimum tenuiflorum 的一种成分,可抑制 SARS-CoV-2 主要蛋白酶的活性和体外病毒复制。

IF 2.5 4区 医学 Q3 CHEMISTRY, MEDICINAL
Hinako Koze, Masayuki Sudoh, Satoaki Onitsuka, Hiroaki Okamura, Takeshi Ishikawa, Fumito Tani, Yukako Miyata-Yabuki, Mikako Shirouzu, Masanori Baba, Mika Okamoto, Toshiyuki Hamada
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引用次数: 0

摘要

新型严重急性呼吸系统综合征冠状病毒 2(SARS-CoV-2)的持续存在和新变异株的出现继续对未来的大流行构成严重威胁。为了应对未来冠状病毒的大流行,迫切需要安全、有效和随时可用的 COVID-19 治疗药物。为了帮助确定新的抗病毒药物,本研究重点研究了圣罗勒(Ocimum tenuiflorum L.)提取物中的天然产物,它们对 SARS-CoV-2 主要蛋白酶(Mpro)具有潜在的抑制作用。在生物测定的指导下,从 O. tenuiflorum 的 MeOH 提取物中分离出了一种含硫甘油糖脂,即磺基喹诺酮酰二酰甘油(SQDG:1),它是一种有效的 Mpro 抑制剂,能有效抑制 Mpro 的活性(IC50:0.42 µM)。SQDG (1) 还能在体外显著抑制 SARS-CoV-2 的复制(EC50,51.2 µM),同时不显示细胞毒性(CC50 > 100 µM)。进一步的抑制动力学研究和对接模拟清楚地表明,SQDG 能以竞争和混合抑制的方式强烈抑制 SARS-CoV-2 Mpro。这些研究结果突出表明,SQDG 是一种有希望用于 COVID-19 治疗的先导化合物,并强调了从天然来源探索新药物的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sulfoquinovosyl diacylglycerol, a component of Holy Basil Ocimum tenuiflorum, inhibits the activity of the SARS-CoV-2 main protease and viral replication in vitro.

The persistence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the emergence of new mutant strains continue to present a substantial threat with potential for future pandemics. Safe, effective, and readily available COVID-19 therapeutics are urgently needed to prepare for future coronavirus pandemics. To help identify new antiviral agents, the present study focused on natural products in the extracts of Holy Basil, Ocimum tenuiflorum L., which show potential inhibitory effects against the SARS-CoV-2 main protease (Mpro). Bioassay-guided isolation of the MeOH extracts of O. tenuiflorum led to the identification of a sulfur-containing glyceroglycolipid, sulfoquinovosyl diacylglycerol (SQDG: 1), as a potent Mpro inhibitor that effectively inhibited Mpro activity (IC50: 0.42 µM). SQDG (1) also markedly suppressed SARS-CoV-2 replication (EC50, 51.2 µM) in vitro while displaying no cytotoxicity (CC50 > 100 µM). Further inhibition kinetic studies and docking simulations clearly demonstrated that SQDG strongly inhibited SARS-CoV-2 Mpro in a competitive and mixed-inhibition manner. These findings highlight SQDG as a promising lead compound for COVID-19 therapy and emphasize the need to explore new drugs from natural sources.

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来源期刊
CiteScore
6.90
自引率
3.00%
发文量
79
审稿时长
1.7 months
期刊介绍: The Journal of Natural Medicines is an international journal publishing original research in naturally occurring medicines and their related foods and cosmetics. It covers: -chemistry of natural products -biochemistry of medicinal plants -pharmacology of natural products and herbs, including Kampo formulas and traditional herbs -botanical anatomy -cultivation of medicinal plants. The journal accepts Original Papers, Notes, Rapid Communications and Natural Resource Letters. Reviews and Mini-Reviews are generally invited.
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