一种基于罗丹明的近红外荧光探针，用于选择性检测 Cu2+ 离子及其在生物成像中的应用。

IF 2.7 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analytical Methods Pub Date : 2024-11-13 DOI:10.1039/D4AY01922A

Zhijian Chang, Shumeng Li, Jia-Hai Ye, Fuyan Lin, Yuncong Chen, Zijian Guo and Xuedong Gong

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引用次数: 0

摘要

新型近红外荧光探针 TM2 基于含罗丹明的框架，具有较大的斯托克斯位移（100 nm）。TM2 在 EtOH/H2O (1 : 1, v/v) 溶液中对 Cu2+ 具有高选择性识别能力，检测限低（2.3 μM），检测范围广（0-50 μM）。在生理 pH 值为 5-9 时，Cu2+ 的检测不受干扰。这种识别机制是由于 TM2 和 Cu2+ 之间形成了 1 ：约伯图、1H NMR 滴定和 LC-MS 实验都验证了这一点。此外，还成功地在体外和体内对 Cu2+ 进行了荧光成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A rhodamine based near-infrared fluorescent probe for selective detection of Cu2+ ions and its applications in bioimaging†

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A rhodamine based near-infrared fluorescent probe for selective detection of Cu2+ ions and its applications in bioimaging†

A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm). TM2 exhibits highly selective recognition for Cu²⁺ in EtOH/H₂O (1 : 1, v/v) solution with a low detection limit (2.3 μM) and a wide detection range (0–50 μM). Detection of Cu²⁺ is undisturbed at physiological pH levels of 5–9. This recognition mechanism is attributed to the formation of a 1 : 1 complex between TM2 and Cu²⁺, validated by Job's plot, ¹H NMR titration, and LC-MS experiments. Moreover, the successful fluorescence imaging of Cu²⁺ both in vitro and in vivo was also accomplished.