Nuocheng Xu , Yutong Feng , Huiping Wan , Zilong Li , Keyu Sun , Shuhong Ye
{"title":"基于复合共凝胶和双乳液结构的益生菌肠道靶向输送系统的构建与表征","authors":"Nuocheng Xu , Yutong Feng , Huiping Wan , Zilong Li , Keyu Sun , Shuhong Ye","doi":"10.1016/j.foodhyd.2024.110814","DOIUrl":null,"url":null,"abstract":"<div><div>The objective of this research was to formulate a sophisticated probiotic intestinal-targeted delivery system employing GEL (Gelatin) and CMC (Carboxymethyl Cellulose) via intricate complex coacervation and double-emulsion structure. RSM (Response Surface Methodology) screening of cryoprotectants was undertaken and the digestive kinetic characteristics of microcapsules were comprehensively evaluated in simulated <em>in vitro</em> release environment. Upon optimization using response surface design, bacterial suspension was prepared using composite cryoprotectant composed of 3.07 wt% MNT (Mannitol), 4.42 wt% TCP (Taxus cuspidata polysaccharides) and 2.01 wt% GEL, achieving a cell recovery rate of 83.21%, which was 70.8% higher than free cells. The freeze-dried microcapsules presented in a stabilized bilayer system, the cell concentration after encapsulation was 8.66 log CFU/g, with encapsulation efficiency reaching 88.17%, and the particle size distribution concentrated at 299.05 μm/d. GEL and CMC conjugate through the interaction of positive and negative ions to form a complex polymer under pH of 3.5. Deprotonation occurs while pH changes in intestinal environment, enabling controlled release. <em>In vitro</em> release kinetics by Hixson-Crowell was closer than Higuchi model, the survival rate of encapsulated cells reached 83.64% in simulated gastric fluid, representing a 56.15% increase compared to free cells. After 6-weeks of storage in low-humidity environment at −18 °C, the recovery rate remained at 94.59%, representing a 69.17% increase compared to free cells. This research offer guiding principles and insights for future comparable research.</div></div>","PeriodicalId":320,"journal":{"name":"Food Hydrocolloids","volume":"160 ","pages":"Article 110814"},"PeriodicalIF":11.0000,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Construction and characterization of probiotic intestinal-targeted delivery system based on complex coacervation and double-emulsion structure\",\"authors\":\"Nuocheng Xu , Yutong Feng , Huiping Wan , Zilong Li , Keyu Sun , Shuhong Ye\",\"doi\":\"10.1016/j.foodhyd.2024.110814\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The objective of this research was to formulate a sophisticated probiotic intestinal-targeted delivery system employing GEL (Gelatin) and CMC (Carboxymethyl Cellulose) via intricate complex coacervation and double-emulsion structure. RSM (Response Surface Methodology) screening of cryoprotectants was undertaken and the digestive kinetic characteristics of microcapsules were comprehensively evaluated in simulated <em>in vitro</em> release environment. Upon optimization using response surface design, bacterial suspension was prepared using composite cryoprotectant composed of 3.07 wt% MNT (Mannitol), 4.42 wt% TCP (Taxus cuspidata polysaccharides) and 2.01 wt% GEL, achieving a cell recovery rate of 83.21%, which was 70.8% higher than free cells. The freeze-dried microcapsules presented in a stabilized bilayer system, the cell concentration after encapsulation was 8.66 log CFU/g, with encapsulation efficiency reaching 88.17%, and the particle size distribution concentrated at 299.05 μm/d. GEL and CMC conjugate through the interaction of positive and negative ions to form a complex polymer under pH of 3.5. Deprotonation occurs while pH changes in intestinal environment, enabling controlled release. <em>In vitro</em> release kinetics by Hixson-Crowell was closer than Higuchi model, the survival rate of encapsulated cells reached 83.64% in simulated gastric fluid, representing a 56.15% increase compared to free cells. After 6-weeks of storage in low-humidity environment at −18 °C, the recovery rate remained at 94.59%, representing a 69.17% increase compared to free cells. This research offer guiding principles and insights for future comparable research.</div></div>\",\"PeriodicalId\":320,\"journal\":{\"name\":\"Food Hydrocolloids\",\"volume\":\"160 \",\"pages\":\"Article 110814\"},\"PeriodicalIF\":11.0000,\"publicationDate\":\"2024-11-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Food Hydrocolloids\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0268005X24010889\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Hydrocolloids","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0268005X24010889","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Construction and characterization of probiotic intestinal-targeted delivery system based on complex coacervation and double-emulsion structure
The objective of this research was to formulate a sophisticated probiotic intestinal-targeted delivery system employing GEL (Gelatin) and CMC (Carboxymethyl Cellulose) via intricate complex coacervation and double-emulsion structure. RSM (Response Surface Methodology) screening of cryoprotectants was undertaken and the digestive kinetic characteristics of microcapsules were comprehensively evaluated in simulated in vitro release environment. Upon optimization using response surface design, bacterial suspension was prepared using composite cryoprotectant composed of 3.07 wt% MNT (Mannitol), 4.42 wt% TCP (Taxus cuspidata polysaccharides) and 2.01 wt% GEL, achieving a cell recovery rate of 83.21%, which was 70.8% higher than free cells. The freeze-dried microcapsules presented in a stabilized bilayer system, the cell concentration after encapsulation was 8.66 log CFU/g, with encapsulation efficiency reaching 88.17%, and the particle size distribution concentrated at 299.05 μm/d. GEL and CMC conjugate through the interaction of positive and negative ions to form a complex polymer under pH of 3.5. Deprotonation occurs while pH changes in intestinal environment, enabling controlled release. In vitro release kinetics by Hixson-Crowell was closer than Higuchi model, the survival rate of encapsulated cells reached 83.64% in simulated gastric fluid, representing a 56.15% increase compared to free cells. After 6-weeks of storage in low-humidity environment at −18 °C, the recovery rate remained at 94.59%, representing a 69.17% increase compared to free cells. This research offer guiding principles and insights for future comparable research.
期刊介绍:
Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication.
The main areas of interest are:
-Chemical and physicochemical characterisation
Thermal properties including glass transitions and conformational changes-
Rheological properties including viscosity, viscoelastic properties and gelation behaviour-
The influence on organoleptic properties-
Interfacial properties including stabilisation of dispersions, emulsions and foams-
Film forming properties with application to edible films and active packaging-
Encapsulation and controlled release of active compounds-
The influence on health including their role as dietary fibre-
Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes-
New hydrocolloids and hydrocolloid sources of commercial potential.
The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.