Gang Xu, Jingyun Zhao, Jieqiong Yao, Yang Xu, Xianghao Yuan, Siyi Pan
{"title":"老化对陈皮多糖精细结构、链构象和形态的影响","authors":"Gang Xu, Jingyun Zhao, Jieqiong Yao, Yang Xu, Xianghao Yuan, Siyi Pan","doi":"10.1016/j.carbpol.2024.122970","DOIUrl":null,"url":null,"abstract":"<div><div>The aging process endows Chenpi (<em>Pericarpium Citri Reticulatae</em>) with unique value and efficacy. This study investigated the dynamic changes in the fine structure, chain conformation, and morphology of water-soluble polysaccharides from Chenpi over time. In the initial storage period of Chenpi (1 year), Chenpi polysaccharides (CP) exhibited a triple-helical structure, with chains entangled and aggregated into rough spherical conformations. Galacturonic acid (GalA, 47.78 mol%) was the predominant monosaccharide. As aging progressed, the homogalacturonan (HG) regions gradually degraded, leading to decreases in molecular sizes (particle size, M<sub>w</sub>, M<sub>n</sub>, and R<sub>g</sub>), a reduction in the degree of esterification, and a weakening of the semicrystalline structure. Consequently, arabinose (Ara) emerged as the main monosaccharide (41.38 mol%). Neutral sugars continuously enriched the rhamnogalacturonan (RG-I) side chains, forming highly branched single molecules that curled into spherical shapes. By 15 years, CP were fully degraded, adopting a compact molecular conformation with the triple-helix structure disappearing and sizes uniformly below 20 nm. However, AFM results indicated aggregation phenomena in 15-year CP. Additionally, CP viscosity decreased while thermal stability improved, reflecting the natural structural transformation of CP. This study provides scientific evidence supporting the application of Chenpi in the food and pharmaceutical industries.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 122970"},"PeriodicalIF":10.7000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of aging on the fine structure, chain conformation, and morphology of Chenpi polysaccharides\",\"authors\":\"Gang Xu, Jingyun Zhao, Jieqiong Yao, Yang Xu, Xianghao Yuan, Siyi Pan\",\"doi\":\"10.1016/j.carbpol.2024.122970\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The aging process endows Chenpi (<em>Pericarpium Citri Reticulatae</em>) with unique value and efficacy. This study investigated the dynamic changes in the fine structure, chain conformation, and morphology of water-soluble polysaccharides from Chenpi over time. In the initial storage period of Chenpi (1 year), Chenpi polysaccharides (CP) exhibited a triple-helical structure, with chains entangled and aggregated into rough spherical conformations. Galacturonic acid (GalA, 47.78 mol%) was the predominant monosaccharide. As aging progressed, the homogalacturonan (HG) regions gradually degraded, leading to decreases in molecular sizes (particle size, M<sub>w</sub>, M<sub>n</sub>, and R<sub>g</sub>), a reduction in the degree of esterification, and a weakening of the semicrystalline structure. Consequently, arabinose (Ara) emerged as the main monosaccharide (41.38 mol%). Neutral sugars continuously enriched the rhamnogalacturonan (RG-I) side chains, forming highly branched single molecules that curled into spherical shapes. By 15 years, CP were fully degraded, adopting a compact molecular conformation with the triple-helix structure disappearing and sizes uniformly below 20 nm. However, AFM results indicated aggregation phenomena in 15-year CP. Additionally, CP viscosity decreased while thermal stability improved, reflecting the natural structural transformation of CP. This study provides scientific evidence supporting the application of Chenpi in the food and pharmaceutical industries.</div></div>\",\"PeriodicalId\":261,\"journal\":{\"name\":\"Carbohydrate Polymers\",\"volume\":\"349 \",\"pages\":\"Article 122970\"},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2024-11-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbohydrate Polymers\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0144861724011962\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Polymers","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0144861724011962","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Effects of aging on the fine structure, chain conformation, and morphology of Chenpi polysaccharides
The aging process endows Chenpi (Pericarpium Citri Reticulatae) with unique value and efficacy. This study investigated the dynamic changes in the fine structure, chain conformation, and morphology of water-soluble polysaccharides from Chenpi over time. In the initial storage period of Chenpi (1 year), Chenpi polysaccharides (CP) exhibited a triple-helical structure, with chains entangled and aggregated into rough spherical conformations. Galacturonic acid (GalA, 47.78 mol%) was the predominant monosaccharide. As aging progressed, the homogalacturonan (HG) regions gradually degraded, leading to decreases in molecular sizes (particle size, Mw, Mn, and Rg), a reduction in the degree of esterification, and a weakening of the semicrystalline structure. Consequently, arabinose (Ara) emerged as the main monosaccharide (41.38 mol%). Neutral sugars continuously enriched the rhamnogalacturonan (RG-I) side chains, forming highly branched single molecules that curled into spherical shapes. By 15 years, CP were fully degraded, adopting a compact molecular conformation with the triple-helix structure disappearing and sizes uniformly below 20 nm. However, AFM results indicated aggregation phenomena in 15-year CP. Additionally, CP viscosity decreased while thermal stability improved, reflecting the natural structural transformation of CP. This study provides scientific evidence supporting the application of Chenpi in the food and pharmaceutical industries.
期刊介绍:
Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience.
The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.