{"title":"首次在反刍动物中发现 Alistipes onderdonkii 并确定其分子特征","authors":"Kyriacos A. Hasapis , Iris Charalambidou , Catherine O.’Dowd Phanis , Stefanie Kazamia , Nicolaos Kassinis , Chad Schou , Panagiotis Karanis","doi":"10.1016/j.microb.2024.100205","DOIUrl":null,"url":null,"abstract":"<div><div><em>Alistipes onderdonkii (A. onderdonkii)</em> is a newly characterised species of bacteria that has been extensively studied in humans as a part of the normal gut microbiota; its role in gastrointestinal diseases and some types of cancer is under debate. In this study <em>A. onderdonkii</em> is detected in animal stools using PCR and sequencing of the PCR products. A total of 146 faecal samples from ruminants, precisely 32 domestic sheep (<em>Ovis aries</em>), 10 domestic and 34 free-ranging goats (<em>Capra hircus</em>) and 70 wild mouflons (<em>Ovis gmelini ophion</em>) were examined, and 2 of them were positive for <em>A. ondendonkii</em> (2/146=1.4 %), both belonging to goats (2/44=4.5 %), one belonging to a domestic goat and the second belonging to a free-ranging goat. To our knowledge, this is the first report of <em>A. onderdonkii</em> in ruminants. This study aimed to evaluate the prevalence of <em>A. onderdonkii</em> in domestic and wild small ruminants in Cyprus. More research is required to elucidate the role of <em>A. onderdonkii</em> in animal health.</div></div>","PeriodicalId":101246,"journal":{"name":"The Microbe","volume":"5 ","pages":"Article 100205"},"PeriodicalIF":0.0000,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"First detection and molecular characterization of Alistipes onderdonkii in ruminants\",\"authors\":\"Kyriacos A. Hasapis , Iris Charalambidou , Catherine O.’Dowd Phanis , Stefanie Kazamia , Nicolaos Kassinis , Chad Schou , Panagiotis Karanis\",\"doi\":\"10.1016/j.microb.2024.100205\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Alistipes onderdonkii (A. onderdonkii)</em> is a newly characterised species of bacteria that has been extensively studied in humans as a part of the normal gut microbiota; its role in gastrointestinal diseases and some types of cancer is under debate. In this study <em>A. onderdonkii</em> is detected in animal stools using PCR and sequencing of the PCR products. A total of 146 faecal samples from ruminants, precisely 32 domestic sheep (<em>Ovis aries</em>), 10 domestic and 34 free-ranging goats (<em>Capra hircus</em>) and 70 wild mouflons (<em>Ovis gmelini ophion</em>) were examined, and 2 of them were positive for <em>A. ondendonkii</em> (2/146=1.4 %), both belonging to goats (2/44=4.5 %), one belonging to a domestic goat and the second belonging to a free-ranging goat. To our knowledge, this is the first report of <em>A. onderdonkii</em> in ruminants. This study aimed to evaluate the prevalence of <em>A. onderdonkii</em> in domestic and wild small ruminants in Cyprus. More research is required to elucidate the role of <em>A. onderdonkii</em> in animal health.</div></div>\",\"PeriodicalId\":101246,\"journal\":{\"name\":\"The Microbe\",\"volume\":\"5 \",\"pages\":\"Article 100205\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Microbe\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2950194624001729\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Microbe","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950194624001729","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
Alistipes onderdonkii（A. onderdonkii）是一种新发现的细菌，作为正常肠道微生物群的一部分，它在人类中的作用已被广泛研究；它在胃肠道疾病和某些类型癌症中的作用还在争论中。本研究利用 PCR 和 PCR 产物测序技术检测动物粪便中的 A. onderdonkii。共检测了 146 份反刍动物粪便样本，包括 32 只家养绵羊（Ovis aries）、10 只家养山羊（Capra hircus）和 34 只散养山羊（Capra hircus）以及 70 只野生毛峰羊（Ovis gmelini ophion），其中 2 份样本对 A. ondendonkii 呈阳性（2/146=1.4 %），两份样本均属于山羊（2/44=4.5 %），一份属于家养山羊，另一份属于散养山羊。据我们所知，这是反刍动物中 A. onderdonkii 的首次报告。本研究旨在评估 A. onderdonkii 在塞浦路斯家养和野生小型反刍动物中的流行情况。需要开展更多研究，以阐明 A. onderdonkii 在动物健康中的作用。
First detection and molecular characterization of Alistipes onderdonkii in ruminants
Alistipes onderdonkii (A. onderdonkii) is a newly characterised species of bacteria that has been extensively studied in humans as a part of the normal gut microbiota; its role in gastrointestinal diseases and some types of cancer is under debate. In this study A. onderdonkii is detected in animal stools using PCR and sequencing of the PCR products. A total of 146 faecal samples from ruminants, precisely 32 domestic sheep (Ovis aries), 10 domestic and 34 free-ranging goats (Capra hircus) and 70 wild mouflons (Ovis gmelini ophion) were examined, and 2 of them were positive for A. ondendonkii (2/146=1.4 %), both belonging to goats (2/44=4.5 %), one belonging to a domestic goat and the second belonging to a free-ranging goat. To our knowledge, this is the first report of A. onderdonkii in ruminants. This study aimed to evaluate the prevalence of A. onderdonkii in domestic and wild small ruminants in Cyprus. More research is required to elucidate the role of A. onderdonkii in animal health.
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