Mariana Mazetto de Carvalho, Christiane Færestrand Ellefsen, Andrea Angelov Eltvik, Marianne Hiorth, Anne Berit C. Samuelsen
{"title":"利用扩散有序核磁共振光谱(DOSY)确定多糖的化学结构特征","authors":"Mariana Mazetto de Carvalho, Christiane Færestrand Ellefsen, Andrea Angelov Eltvik, Marianne Hiorth, Anne Berit C. Samuelsen","doi":"10.1016/j.carbpol.2024.123021","DOIUrl":null,"url":null,"abstract":"<div><div>The potential of DOSY NMR spectroscopy to distinguish the linkage pattern of chemically related polysaccharides was evaluated using β-glucans isolated from yeast (<em>Saccharomyces cerevisiae</em>) and mushroom (<em>Pleurotus eryngii</em>). Laminarin from <em>Laminaria digitata</em> was included for chemical shift comparison. Characterization through methylation and 1D/2D NMR analysis showed that all the samples were constituted by <span><math><mo>→</mo></math></span>3)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span>; →3,6)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span>; Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> and <span><math><mo>→</mo></math></span>6)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> linkages. The results obtained allowed the identification of the well-known chemical structure of laminarin. Moreover, DOSY demonstrated that the units <span><math><mo>→</mo></math></span>3)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> and <span><math><mo>→</mo></math></span>6)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> from yeast β-glucans, presented the same diffusion time. For the mushrooms β-glucans, the diffusion time of these units were different, confirming that they belong to distinct polysaccharides. The yeast and the mushroom polysaccharide samples presented the same NMR correlations, but after DOSY analysis, different structures could be proposed. Therefore, DOSY NMR spectroscopy could be a tool for the identification of different linkage patterns of polysaccharides belonging to the same group and may be a useful contribution to the chemical structure and biological activity correlation studies of such structures.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123021"},"PeriodicalIF":10.7000,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Chemical structure characterization of polysaccharides using diffusion ordered NMR spectroscopy (DOSY)\",\"authors\":\"Mariana Mazetto de Carvalho, Christiane Færestrand Ellefsen, Andrea Angelov Eltvik, Marianne Hiorth, Anne Berit C. Samuelsen\",\"doi\":\"10.1016/j.carbpol.2024.123021\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The potential of DOSY NMR spectroscopy to distinguish the linkage pattern of chemically related polysaccharides was evaluated using β-glucans isolated from yeast (<em>Saccharomyces cerevisiae</em>) and mushroom (<em>Pleurotus eryngii</em>). Laminarin from <em>Laminaria digitata</em> was included for chemical shift comparison. Characterization through methylation and 1D/2D NMR analysis showed that all the samples were constituted by <span><math><mo>→</mo></math></span>3)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span>; →3,6)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span>; Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> and <span><math><mo>→</mo></math></span>6)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> linkages. The results obtained allowed the identification of the well-known chemical structure of laminarin. Moreover, DOSY demonstrated that the units <span><math><mo>→</mo></math></span>3)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> and <span><math><mo>→</mo></math></span>6)-Glc<em>p</em>-(1<span><math><mo>→</mo></math></span> from yeast β-glucans, presented the same diffusion time. For the mushrooms β-glucans, the diffusion time of these units were different, confirming that they belong to distinct polysaccharides. The yeast and the mushroom polysaccharide samples presented the same NMR correlations, but after DOSY analysis, different structures could be proposed. Therefore, DOSY NMR spectroscopy could be a tool for the identification of different linkage patterns of polysaccharides belonging to the same group and may be a useful contribution to the chemical structure and biological activity correlation studies of such structures.</div></div>\",\"PeriodicalId\":261,\"journal\":{\"name\":\"Carbohydrate Polymers\",\"volume\":\"349 \",\"pages\":\"Article 123021\"},\"PeriodicalIF\":10.7000,\"publicationDate\":\"2024-11-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Carbohydrate Polymers\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0144861724012475\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Polymers","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0144861724012475","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Chemical structure characterization of polysaccharides using diffusion ordered NMR spectroscopy (DOSY)
The potential of DOSY NMR spectroscopy to distinguish the linkage pattern of chemically related polysaccharides was evaluated using β-glucans isolated from yeast (Saccharomyces cerevisiae) and mushroom (Pleurotus eryngii). Laminarin from Laminaria digitata was included for chemical shift comparison. Characterization through methylation and 1D/2D NMR analysis showed that all the samples were constituted by 3)-Glcp-(1; →3,6)-Glcp-(1; Glcp-(1 and 6)-Glcp-(1 linkages. The results obtained allowed the identification of the well-known chemical structure of laminarin. Moreover, DOSY demonstrated that the units 3)-Glcp-(1 and 6)-Glcp-(1 from yeast β-glucans, presented the same diffusion time. For the mushrooms β-glucans, the diffusion time of these units were different, confirming that they belong to distinct polysaccharides. The yeast and the mushroom polysaccharide samples presented the same NMR correlations, but after DOSY analysis, different structures could be proposed. Therefore, DOSY NMR spectroscopy could be a tool for the identification of different linkage patterns of polysaccharides belonging to the same group and may be a useful contribution to the chemical structure and biological activity correlation studies of such structures.
期刊介绍:
Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience.
The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.