Evolving CRISPR/Cas system for food safety monitoring across the food supply chain

The rapid detection methods are regarded as one of the most important ways to ensure food safety across the food supply chain worldwide. While the limitations including low sensitivity, poor portability and unsatisfactory accuracy still remain. The emerging CRISPR/Cas system, a powerful tool in gene editing and biosensing, possesses high specificity and sensitivity, showing great potential for food safety detection. This review comprehensively summarizes the CRISPR/Cas-based biosensing and its applications in food safety monitoring, spanning from the foundational principles to recent advancements. Divided from the detection requirements perspective, the technical development of CRISPR-Sensor is going through the stages from satisfying basic detection needs to pursuing better sensing performance. With the rapid development of artificial intelligence, the CRISPR-Sensor is empowered to revolutionize biosensing, offering more possibilities for continuous monitoring and accurate prediction. Lastly, the current challenges and future prospects of the CRISPR-Sensor for food safety monitoring are illustrated.