利用分子印迹聚合物涂层木尖电喷雾离子化质谱法快速灵敏地测定邦克瑞克酸

IF 5.2 Q1 CHEMISTRY, ANALYTICAL
Chunfei Zhong , Xinyan Li , Fan Zhang , Ning Liu , Jiewei Deng , Yunyun Yang , Tiangang Luan
{"title":"利用分子印迹聚合物涂层木尖电喷雾离子化质谱法快速灵敏地测定邦克瑞克酸","authors":"Chunfei Zhong ,&nbsp;Xinyan Li ,&nbsp;Fan Zhang ,&nbsp;Ning Liu ,&nbsp;Jiewei Deng ,&nbsp;Yunyun Yang ,&nbsp;Tiangang Luan","doi":"10.1016/j.sampre.2024.100137","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, a novel methodology has been developed for the rapid and sensitive analysis of bongkrekic acid (BA), a highly toxic bacterial metabolite, using a molecularly imprinted polymer-coated wooden-tip coupled with electrospray ionization mass spectrometry (MIPWT-ESI-MS). A MIPWT solid phase microextraction (SPME) probe specifically tailored for BA was fabricated, by employing dummy template molecules/functional monomers in the imprinting coating modification. This approach exhibits an enrichment factor (EF) of 683 ± 53 for efficient extraction of BA from aqueous matrices, demonstrating the utility of dummy templates in enhancing the selectivity and sensitivity of the MIPWT-ESI-MS method. The analytical performance of the MIPWT-ESI-MS method was evaluated, demonstrating a desirable linear correlation (<em>r</em> = 0.9988), along with remarkable sensitivity by a limit of detection (LOD) and limit of quantitation (LOQ) at 0.05 μg/L and 0.16 μg/L in pure water, respectively. Additionally, the method demonstrated high repeatability with a relative standard deviation (RSD) &lt; 3.9 % (<em>n</em> = 6) and reproducibility with an RSD &lt; 13.4 %. The practical application of the method was confirmed through the successful analysis of real-world samples, including tap water, wastewater from a food processing factory, and the aqueous extracts of food samples, with BA detected at 0.296 µg/L in <em>Tremella</em>. Spiking experiments further validated the accuracy of the method, with recoveries ranging from 81 % to 97 %. This study integrates MIP with ambient mass spectrometry, offering a robust, selective, and sensitive tool for the monitoring of trace BA levels in complex food and environmental samples, contributing significantly to public health safety and food quality control.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"12 ","pages":"Article 100137"},"PeriodicalIF":5.2000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Rapid and sensitive determination of bongkrekic acid with molecularly imprinted polymer-coated wooden-tip electrospray ionization mass spectrometry\",\"authors\":\"Chunfei Zhong ,&nbsp;Xinyan Li ,&nbsp;Fan Zhang ,&nbsp;Ning Liu ,&nbsp;Jiewei Deng ,&nbsp;Yunyun Yang ,&nbsp;Tiangang Luan\",\"doi\":\"10.1016/j.sampre.2024.100137\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In this study, a novel methodology has been developed for the rapid and sensitive analysis of bongkrekic acid (BA), a highly toxic bacterial metabolite, using a molecularly imprinted polymer-coated wooden-tip coupled with electrospray ionization mass spectrometry (MIPWT-ESI-MS). A MIPWT solid phase microextraction (SPME) probe specifically tailored for BA was fabricated, by employing dummy template molecules/functional monomers in the imprinting coating modification. This approach exhibits an enrichment factor (EF) of 683 ± 53 for efficient extraction of BA from aqueous matrices, demonstrating the utility of dummy templates in enhancing the selectivity and sensitivity of the MIPWT-ESI-MS method. The analytical performance of the MIPWT-ESI-MS method was evaluated, demonstrating a desirable linear correlation (<em>r</em> = 0.9988), along with remarkable sensitivity by a limit of detection (LOD) and limit of quantitation (LOQ) at 0.05 μg/L and 0.16 μg/L in pure water, respectively. Additionally, the method demonstrated high repeatability with a relative standard deviation (RSD) &lt; 3.9 % (<em>n</em> = 6) and reproducibility with an RSD &lt; 13.4 %. The practical application of the method was confirmed through the successful analysis of real-world samples, including tap water, wastewater from a food processing factory, and the aqueous extracts of food samples, with BA detected at 0.296 µg/L in <em>Tremella</em>. Spiking experiments further validated the accuracy of the method, with recoveries ranging from 81 % to 97 %. This study integrates MIP with ambient mass spectrometry, offering a robust, selective, and sensitive tool for the monitoring of trace BA levels in complex food and environmental samples, contributing significantly to public health safety and food quality control.</div></div>\",\"PeriodicalId\":100052,\"journal\":{\"name\":\"Advances in Sample Preparation\",\"volume\":\"12 \",\"pages\":\"Article 100137\"},\"PeriodicalIF\":5.2000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Sample Preparation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2772582024000354\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Sample Preparation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772582024000354","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

本研究开发了一种新方法,利用分子印迹聚合物涂层木质吸头耦合电喷雾离子化质谱(MIPWT-ESI-MS)快速灵敏地分析剧毒细菌代谢物邦克瑞克酸(BA)。通过在印迹涂层修饰中使用假模板分子/功能单体,制造出了专门针对 BA 的 MIPWT 固相微萃取 (SPME) 探针。这种方法的富集因子(EF)为 683 ± 53,可从水性基质中高效萃取 BA,证明了假模板在提高 MIPWT-ESI-MS 方法的选择性和灵敏度方面的实用性。对 MIPWT-ESI-MS 方法的分析性能进行了评估,结果表明该方法具有理想的线性相关性(r = 0.9988),灵敏度高,在纯水中的检出限(LOD)和定量限(LOQ)分别为 0.05 μg/L 和 0.16 μg/L。此外,该方法的重复性很高,相对标准偏差(RSD)为 3.9%(n = 6),重现性为 13.4%。通过对实际样品(包括自来水、食品加工厂的废水和食品样品的水提取物)的成功分析,证实了该方法的实际应用性,在 Tremella 中检测到的 BA 为 0.296 µg/L。加标实验进一步验证了该方法的准确性,回收率在 81 % 到 97 % 之间。这项研究将 MIP 与环境质谱法相结合,为监测复杂食品和环境样品中的痕量 BA 含量提供了一种稳健、选择性强且灵敏的工具,对公共卫生安全和食品质量控制大有裨益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Rapid and sensitive determination of bongkrekic acid with molecularly imprinted polymer-coated wooden-tip electrospray ionization mass spectrometry

Rapid and sensitive determination of bongkrekic acid with molecularly imprinted polymer-coated wooden-tip electrospray ionization mass spectrometry
In this study, a novel methodology has been developed for the rapid and sensitive analysis of bongkrekic acid (BA), a highly toxic bacterial metabolite, using a molecularly imprinted polymer-coated wooden-tip coupled with electrospray ionization mass spectrometry (MIPWT-ESI-MS). A MIPWT solid phase microextraction (SPME) probe specifically tailored for BA was fabricated, by employing dummy template molecules/functional monomers in the imprinting coating modification. This approach exhibits an enrichment factor (EF) of 683 ± 53 for efficient extraction of BA from aqueous matrices, demonstrating the utility of dummy templates in enhancing the selectivity and sensitivity of the MIPWT-ESI-MS method. The analytical performance of the MIPWT-ESI-MS method was evaluated, demonstrating a desirable linear correlation (r = 0.9988), along with remarkable sensitivity by a limit of detection (LOD) and limit of quantitation (LOQ) at 0.05 μg/L and 0.16 μg/L in pure water, respectively. Additionally, the method demonstrated high repeatability with a relative standard deviation (RSD) < 3.9 % (n = 6) and reproducibility with an RSD < 13.4 %. The practical application of the method was confirmed through the successful analysis of real-world samples, including tap water, wastewater from a food processing factory, and the aqueous extracts of food samples, with BA detected at 0.296 µg/L in Tremella. Spiking experiments further validated the accuracy of the method, with recoveries ranging from 81 % to 97 %. This study integrates MIP with ambient mass spectrometry, offering a robust, selective, and sensitive tool for the monitoring of trace BA levels in complex food and environmental samples, contributing significantly to public health safety and food quality control.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.50
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信