优化纤维酵母菌 Y1402 在烟草基质中生产 3-甲硫基-1-丙醇的发酵条件

IF 1.2 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES
Qi Sun , Xiaoyan Liu , Quanyu Zhou , Xinhui Peng , Xuemin Guo , Bo Kong , Huawu Yang , Chao Teng , Liujie Cheng , Chan Zhang , Zhen Ma , Guangsen Fan
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引用次数: 0

摘要

3-甲硫基-1-丙醇(3-Met)被广泛用作食品香料中的调味物质。本研究优化了纤维酵母菌 Y1402 在烟草基质中生产 3-Met 的发酵条件。首先,通过单因素设计对培养基成分和培养条件进行了优化。然后,采用普拉克特-伯曼设计(Plackett-Burman design)筛选出对纤维菌 Y1402 产生 3-Met 影响最大的因素。采用最陡上升路径设计来确定最重要的响应区域。利用箱-贝肯实验设计和响应面分析法确定了生产 3-Met 的最佳条件。这些条件包括:烟草的粒度在 60 至 80 目之间;硫酸铵作为氮源,浓度为 2.4 克/升;营养液中的 L-蛋氨酸(L-Met)浓度为 3.1 克/升;培养时间为 145 小时;含水量为 83%;初始 pH 值为 5.5;温度为 29 °C;接种量为 1.6%。在这些条件下,3-Met 的产量达到每克烟草 342.07 毫克。当扩大培养规模时,3-甲基甲烷的转化率达到 1.07 克/克烟草。这表明,发酵这种菌株可以增加烟叶中 3-Met 的含量。这项研究为利用该菌株提高碎烟叶、劣质烟叶或烟梗的风味成分提供了参考价值,有利于劣质烟叶的加工。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of fermentation conditions for 3-methylthio-1-propanol production by Saccharomycopsis fibuligera Y1402 in tobacco matrix
3-methylthio-1-propanol (3-Met) is widely used as a flavoring substance in food flavoring. In this study, the fermentation conditions of Saccharomycopsis fibuligera Y1402 for 3-Met production were optimized in the tobacco matrix. Firstly, the optimization of the culture medium components and culture conditions was carried out through a single factor design. Then, a Plackett-Burman design was used to screen out factors that most significantly affected the production of 3-Met by S. fibuligera Y1402. The steepest ascending path design was used to identify the most significant response region. The optimal conditions for the production of 3-Met were determined using the Box-Behnken experimental design and response surface analysis. These conditions included a particle size of tobacco between 60 and 80 mesh, ammonium sulphate as the nitrogen source with a concentration of 2.4 g/L, L-methionine (L-Met) concentration of 3.1 g/L in the nutrient solution, an incubation time of 145 h, a moisture content of 83%, an initial pH of 5.5, a temperature of 29 °C, and an inoculation size of 1.6%. Under these conditions, the production of 3-Met reached 342.07 mg/g of tobacco. When the culture was scaled up, the conversion yield of 3-Met reached 1.07 g/g of tobacco. This suggests that fermenting this strain can increase the amount of 3-Met in tobacco leaves. This research offers a reference value for employing this strain to enhance the flavor components of crushed tobacco leaves, low-quality tobacco leaves, or tobacco stems, facilitating the processing of inferior tobacco leaves.
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来源期刊
Kuwait Journal of Science
Kuwait Journal of Science MULTIDISCIPLINARY SCIENCES-
CiteScore
1.60
自引率
28.60%
发文量
132
期刊介绍: Kuwait Journal of Science (KJS) is indexed and abstracted by major publishing houses such as Chemical Abstract, Science Citation Index, Current contents, Mathematics Abstract, Micribiological Abstracts etc. KJS publishes peer-review articles in various fields of Science including Mathematics, Computer Science, Physics, Statistics, Biology, Chemistry and Earth & Environmental Sciences. In addition, it also aims to bring the results of scientific research carried out under a variety of intellectual traditions and organizations to the attention of specialized scholarly readership. As such, the publisher expects the submission of original manuscripts which contain analysis and solutions about important theoretical, empirical and normative issues.
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