Construction of dual-output molecular logic circuit based on bovine serum albumin loaded with two fluorescent compounds

Most fluorescent compounds have only one fluorescence emission peak, it is difficult to directly construct double-output or triple-output molecular logic devices using a single fluorescent compound. Therefore, this paper proposes using bovine serum albumin (BSA) as a carrier to load 5-sulfosalicylic acid and fluorescein isothiocyanate at different sites of BSA to synthesize the L₁/L₂@BSA complex, and based on fluorescence resonance and electron exchange energy transfer (or hybrid FRET-DET) to construct a dual-output molecular logic circuit. This construction of double-output molecular logic devices based on the combination of two fluorescent compounds using BSA as a carrier can not only perform controllable screening and combination according to the requirements of excitation wavelength or emission wavelength, but perhaps also build an ideal research model for exploring the energy transfer mechanism of multi-molecular coupled exciton fields.