生物活性玻璃的磷灰石形成能力--在人体血清和 Kokubo 模拟体液中的比较研究

IF 5.1 2区材料科学 Q1 MATERIALS SCIENCE, CERAMICS

Ceramics International Pub Date : 2024-10-02 DOI:10.1016/j.ceramint.2024.10.013

Adrian Szewczyk , Adrianna Skwira-Rucińska , Marta Osińska , Magdalena Prokopowicz

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引用次数: 0

摘要

目前，对生物材料磷灰石形成能力的评估采用 Kokubo 的模拟体液（SBF）作为测试介质。由于传统的 SBF 仅模拟人血清（HS）的离子成分和 pH 值，本研究旨在比较在 SBF 和 HS 中溶胶凝胶衍生生物玻璃（BG）83S2 和商用 45S5 的磷灰石形成情况，并验证 BG 在这两种液体中的预培养对人类胎儿成骨细胞系 hFOB 1.19 的细胞相容性的影响。无论使用哪种 BG，HS 中磷灰石的形成都会受到阻碍。BG83S2 的磷灰石形成能力相对较高。在直接接触试验中，无论使用哪种液体，两种 BG 对成骨细胞都具有细胞相容性。然而，就提取物而言，只有在 HS 中预培养的 BG83S2 提取物没有显示出细胞毒性效应。在 SBF 中评估磷灰石形成能力似乎是比较各种生物类磷灰石形成能力的一种快速筛选方法。不过，SBF 无法精确模仿在 HS 中检测时观察到的磷灰石形态和结晶度，也无法模仿成骨离子的特征变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

The apatite-forming ability of bioactive glasses – A comparative study in human serum and Kokubo's simulated body fluid

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The apatite-forming ability of bioactive glasses – A comparative study in human serum and Kokubo's simulated body fluid

Currently, the apatite-forming ability evaluation of a biomaterial employs Kokubo's simulated body fluid (SBF) as a testing medium. As the conventional SBF simulates only the ionic composition and pH of human serum (HS), this study aimed to compare the apatite formation in SBF and HS on the sol-gel-derived bioglass (BG) 83S2 and commercial 45S5 as well as to verify the influence of preincubation of BG in both fluids on cytocompatibility towards human fetal osteoblast cell line hFOB 1.19. The apatite formation in HS was hindered, regardless of the type of used BG. Relatively higher apatite-forming ability was noticed for BG83S2. In direct contact test, both BGs were cytocompatible towards osteoblasts, regardless of the type of used fluid. In the case of extracts; however, only the extract obtained from BG83S2 preincubated in HS did not show the cytotoxic effect. The evaluation of apatite-forming ability in SBF appears to be a fast screening method for comparing the apatite-forming ability of various bioglasses. Nonetheless, SBF is unable to precisely imitate the morphology and crystallinity of apatite observed during the assay in HS, as well as it does not mimic the changes in profiles of osteogenic ions.

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来源期刊

Ceramics International 工程技术-材料科学：硅酸盐

CiteScore

9.40

自引率

15.40%

发文量

4558

审稿时长

25 days

期刊介绍： Ceramics International covers the science of advanced ceramic materials. The journal encourages contributions that demonstrate how an understanding of the basic chemical and physical phenomena may direct materials design and stimulate ideas for new or improved processing techniques, in order to obtain materials with desired structural features and properties. Ceramics International covers oxide and non-oxide ceramics, functional glasses, glass ceramics, amorphous inorganic non-metallic materials (and their combinations with metal and organic materials), in the form of particulates, dense or porous bodies, thin/thick films and laminated, graded and composite structures. Process related topics such as ceramic-ceramic joints or joining ceramics with dissimilar materials, as well as surface finishing and conditioning are also covered. Besides traditional processing techniques, manufacturing routes of interest include innovative procedures benefiting from externally applied stresses, electromagnetic fields and energetic beams, as well as top-down and self-assembly nanotechnology approaches. In addition, the journal welcomes submissions on bio-inspired and bio-enabled materials designs, experimentally validated multi scale modelling and simulation for materials design, and the use of the most advanced chemical and physical characterization techniques of structure, properties and behaviour. Technologically relevant low-dimensional systems are a particular focus of Ceramics International. These include 0, 1 and 2-D nanomaterials (also covering CNTs, graphene and related materials, and diamond-like carbons), their nanocomposites, as well as nano-hybrids and hierarchical multifunctional nanostructures that might integrate molecular, biological and electronic components.