用于快速硬脑膜再生的梯度涂层径向结构支架：提供快速迁移路径和强大的迁移动力

IF 12.7 1区材料科学 Q1 ENGINEERING, MULTIDISCIPLINARY

Composites Part B: Engineering Pub Date : 2024-11-13 DOI:10.1016/j.compositesb.2024.111975

Min Qi , Jie Liao , Bingbing Wang , Wei He , Shuyan Liu , Shuyu Liu , Yuntao Di , Qiang Cai , Zhiwei Xu , Xiaoming Li

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引用次数: 0

摘要

由于天然硬脑膜的独特功能，有必要设计具有多层结构的硬脑膜修复体，以实现防渗漏、防粘连和促再生的多功能性。由于防渗漏或防粘连层对细胞具有屏障作用，促再生层主要通过诱导缺损边缘的脑膜成纤维细胞来修复硬脑膜缺损。因此，构建能提供快速、笔直迁移路径和强大迁移动力的支架是实现快速缺损修复的关键。本研究设计并构建了一种涂有浓度梯度无细胞小肠粘膜（SIS）的激进结构支架。该支架提供了一个宽度约为 75 μm 的直通道，适合细胞侵袭和 SIS 涂层引起的强大迁移动力。体外和体内实验均证明，与随机结构的支架相比，该支架在促进细胞侵袭和组织再生方面具有更优越的功效。具体来说，植入后第 14 天，支架中的细胞迁移量、植入后第 28 天的胶原沉积量和血管生成量分别提高了 4.43 倍、0.51 倍和 2.61 倍。在涂覆 SIS 后，这些增强效果得到了进一步改善。因此，这种涂有浓度梯度 SIS 的激元结构支架有望促进硬脑膜的快速再生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Gradient-coated radial-structured scaffolds for rapid dural regeneration: Providing fast migration pathways and strong migration dynamics

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Gradient-coated radial-structured scaffolds for rapid dural regeneration: Providing fast migration pathways and strong migration dynamics

The unique functions of the natural dura mater necessitate the design of dural restorations with a multilayered structure to achieve multifunctionality of anti-leakage, anti-adhesion, and pro-regenerative. Due to the barrier effect of the anti-leakage or anti-adhesion layer on cells, the pro-regenerative layer repairs dural defects mainly by inducing meningeal fibroblasts at the edge of defects. Hence, constructing scaffolds that provide fast and straight migration pathways and strong migration dynamics is the key to achieving fast defect repair. In this study, a radical-structured scaffold coated with concentration-gradient acellular small intestinal submucosa (SIS) is designed and constructed. The scaffold provides a straight channel with about 75 μm of width suitable for cell invasion and strong migration dynamics caused by SIS coating. Both in vitro and in vivo experiments demonstrate its superior efficacy in promoting cellular invasion and tissue regeneration compared to random-structured scaffold. Specifically, cell migration in the scaffolds at day 14 after implantation, and collagen deposition and angiogenesis at day 28 after implantation were elevated 4.43-, 0.51-, and 2.61-fold, respectively. These enhancement effects were further improved after being coated with SIS. Consequently, this radical-structured scaffold coated with concentration-gradient SIS is promising for promoting rapid dural regeneration.

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来源期刊

Composites Part B: Engineering 工程技术-材料科学：复合

CiteScore

24.40

自引率

11.50%

发文量

784

审稿时长

21 days

期刊介绍： Composites Part B: Engineering is a journal that publishes impactful research of high quality on composite materials. This research is supported by fundamental mechanics and materials science and engineering approaches. The targeted research can cover a wide range of length scales, ranging from nano to micro and meso, and even to the full product and structure level. The journal specifically focuses on engineering applications that involve high performance composites. These applications can range from low volume and high cost to high volume and low cost composite development. The main goal of the journal is to provide a platform for the prompt publication of original and high quality research. The emphasis is on design, development, modeling, validation, and manufacturing of engineering details and concepts. The journal welcomes both basic research papers and proposals for review articles. Authors are encouraged to address challenges across various application areas. These areas include, but are not limited to, aerospace, automotive, and other surface transportation. The journal also covers energy-related applications, with a focus on renewable energy. Other application areas include infrastructure, off-shore and maritime projects, health care technology, and recreational products.