用于间充质干细胞软骨分化的热磁响应注射水凝胶

IF 5.5 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications Pub Date : 2024-11-13 DOI:10.1016/j.bioadv.2024.214115

Parvin Najafi , Elnaz Tamjid , Parviz Abdolmaleki , Mehrdad Behmanesh

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引用次数: 0

摘要

由于软骨组织无血管且细胞数量少，受损软骨组织的自我修复能力有限。为了有效地改造软骨组织，需要采用创新技术，如采用微创方法的可注射和交互式水凝胶，以模拟软骨的天然特性。本研究合理设计了一种含有磁性氧化铁纳米颗粒（MNPs）的可注射水凝胶，利用外部磁场诱导骨髓间充质干细胞（BMSCs）进行软骨分化。研究了加入表面官能团为羧基或胺的 MNPs 对水凝胶（分别称为 HS 样品和 HA 样品）性质的影响，并与不含 MNPs 的对照水凝胶（称为 H 样品）进行了比较。这些水凝胶具有热磁响应和剪切稀化行为。在水凝胶组合中加入 MNPs 可形成更坚固的网络，使 HS 和 HA 的压缩模量（分别增加 2 倍和 2.5 倍）、细胞存活率（分别增加 24% 和 7%）、溶胀率（分别增加 97% 和 42%）以及细胞粘附性更好。此外，加入 MNPs 后，HS 和 HA 的弹性模量（分别降低 28 倍和 5 倍）、生物降解率（分别降低 5% 和 9%）和粘度（分别降低 4 倍和 20 倍）均有所降低。糖胺聚糖（GAG）染色结果表明，MNP 的加入和磁场的应用在体外改善 BMSCs 软骨分化方面具有协同效应。这些研究成果将有助于开发用于组织工程应用的优质可注射水凝胶和生物墨水。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Thermomagneto-responsive injectable hydrogel for chondrogenic differentiation of mesenchymal stem cells

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Thermomagneto-responsive injectable hydrogel for chondrogenic differentiation of mesenchymal stem cells

Damaged cartilage tissue has a limited ability to self-heal due to its avascular nature and low cellularity. To effectively engineer cartilage tissue, innovative techniques such as injectable and interactive hydrogels using a minimally invasive approach are required to mimic the natural properties of cartilage. In this study, an injectable hydrogel containing magnetic iron oxide nanoparticles (MNPs) has been rationally designed to induce chondrogenic differentiation in bone marrow mesenchymal stem cells (BMSCs) using an external magnetic field application. The effect of the incorporation of MNPs with the surface functional group of either carboxyl or amine on the properties of the hydrogels (denoted as HS and HA samples, respectively) has been investigated, and compared to control hydrogel without MNPs (denoted as H). The hydrogels demonstrated thermomagnetic-responsive and shear-thinning behavior. Incorporating MNPs in the hydrogel combination resulted in the formation of a more robust network with increased compressive modulus (by 2 and 2.5 times), cell viability (by 24 % and 7 %), swelling ratio (by 97 % and 42 %) for HS and HA, respectively, as well as better cell adhesion. Also, incorporating MNPs resulted in decreased elastic modulus (by 28 and 5 times), biodegradation rate (by 5 % and 9 %), and viscosity (by 4 and 20 times) for HS and HA, respectively. The results of glycosaminoglycans (GAG) staining indicated the synergistic effect of MNP incorporation and magnetic field application in improving chondrogenic differentiation of BMSCs in vitro. The research findings could lead to the development of superior injectable hydrogels and bioinks for tissue engineering applications.

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来源期刊

Materials Science & Engineering C-Materials for Biological Applications 工程技术-材料科学：生物材料

CiteScore

17.80

自引率

0.00%

发文量

501

审稿时长

27 days

期刊介绍： Biomaterials Advances, previously known as Materials Science and Engineering: C-Materials for Biological Applications (P-ISSN: 0928-4931, E-ISSN: 1873-0191). Includes topics at the interface of the biomedical sciences and materials engineering. These topics include: • Bioinspired and biomimetic materials for medical applications • Materials of biological origin for medical applications • Materials for "active" medical applications • Self-assembling and self-healing materials for medical applications • "Smart" (i.e., stimulus-response) materials for medical applications • Ceramic, metallic, polymeric, and composite materials for medical applications • Materials for in vivo sensing • Materials for in vivo imaging • Materials for delivery of pharmacologic agents and vaccines • Novel approaches for characterizing and modeling materials for medical applications Manuscripts on biological topics without a materials science component, or manuscripts on materials science without biological applications, will not be considered for publication in Materials Science and Engineering C. New submissions are first assessed for language, scope and originality (plagiarism check) and can be desk rejected before review if they need English language improvements, are out of scope or present excessive duplication with published sources. Biomaterials Advances sits within Elsevier''s biomaterials science portfolio alongside Biomaterials, Materials Today Bio and Biomaterials and Biosystems. As part of the broader Materials Today family, Biomaterials Advances offers authors rigorous peer review, rapid decisions, and high visibility. We look forward to receiving your submissions!