Delphinidin通过AMPK信号通路在C2C12肌管中诱导快慢肌纤维类型转变

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2024-11-23 DOI:10.1016/j.bbrep.2024.101884

Motoki Murata , Rina Takahashi , Yuki Marugame , Yoshinori Fujimura , Hirofumi Tachibana

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引用次数: 0

摘要

Delphinidin是一种植物花青素，可抑制小鼠废用性肌肉萎缩。然而，它对肌肉纤维类型转变的影响尚不清楚。为了研究Delphinidin是否会影响骨骼肌纤维类型，我们用Delphinidin处理了分化的C2C12细胞。结果发现，delphinidin能上调C2C12肌管中肌球蛋白重链I型（MyHCI）、肌钙蛋白C1、肌钙蛋白I1和MyHCIIx的mRNA表达，并能提高慢速MyHC蛋白水平。Delphinidin还能增强琥珀酸脱氢酶（SDH）活性，抑制乳酸脱氢酶（LDH）活性。抑制单磷酸腺苷激活蛋白激酶（AMPK）可减轻delphinidin诱导的MyHCI上调和MyHCIIb下调。我们研究了delphinidin对参与AMPK激活的上游因子的影响。Delphinidin增加了肝激酶B1（LKB1）的磷酸化以及核呼吸因子1（NRF1）和钙/钙调蛋白依赖性蛋白激酶2（CaMKK2）的蛋白水平。总之，delphinidin 通过 AMPK 信号通路诱导肌肉纤维类型从快肌到慢肌的转换。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Delphinidin induces a fast-to-slow muscle fiber type shift through the AMPK signaling pathway in C2C12 myotubes

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Delphinidin induces a fast-to-slow muscle fiber type shift through the AMPK signaling pathway in C2C12 myotubes

Delphinidin, a plant anthocyanidin, suppresses disuse muscle atrophy in mice. However, its effect on muscle fiber type shift is unclear. To examine whether delphinidin affects skeletal muscle fiber type, differentiated C2C12 cells were treated with delphinidin. Results revealed that delphinidin upregulated the mRNA expression of myosin heavy chain type I (MyHCI), troponin C1, troponin I1, and MyHCIIx and increased slow MyHC protein level in C2C12 myotubes. Delphinidin also enhanced succinic dehydrogenase (SDH) activities and suppressed lactate dehydrogenase (LDH) activity. Adenosine monophosphate–activated protein kinase (AMPK) inhibition attenuated delphinidin-induced MyHCI upregulation and MyHCIIb downregulation. We investigated the effect of delphinidin on the upstream factors involved in AMPK activation. Delphinidin increased liver kinase B1 (LKB1) phosphorylation and nuclear respiratory factor 1 (NRF1) and calcium/calmodulin-dependent protein kinase 2 (CaMKK2) protein levels. In conclusion, delphinidin induced muscle fiber type conversion from fast-twitch to slow-twitch muscles through the AMPK signaling pathway.

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.