合理整合多巴胺的荧光活性和淬灭活性，实现酪胺的反向比率测量和半定量视觉检测

IF 4.1 3区工程技术 Q2 CHEMISTRY, APPLIED

Dyes and Pigments Pub Date : 2024-11-20 DOI:10.1016/j.dyepig.2024.112559

Mingyue You , Lingling Liu , Yuanmin Gong , Rui Shi , Long Yuan , Jiaqi Chen , Kai Zhou , Xueping Tao , Gaoyi Yi , Zhining Xia , Qifeng Fu

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引用次数: 0

摘要

我们开发了一种简便高效的反向比率荧光（RF）方法，用于灵敏准确地检测发酵或陈化食品中的酪胺（Tyr），酪胺是食品腐败的一个重要标志。该方法采用 "活化-淬灭一体化 "策略，涉及酪氨酸的酪氨酸酶催化产物多巴胺（DA）的荧光反应性和淬灭能力。在碱性条件下，DA 与间苯二酚反应生成氮杂蒙那定，发出强烈的蓝色光。此外，DA 还会被 TYR 氧化成邻多巴醌，从而淬灭碲化镉量子点（QD）的红色荧光。在优化条件下，建立了酪氨酸特异性射频传感器，其线性检测范围为 0.1 至 6 mg/mL，检测限为 8.50 × 10-3 mg/L。制备的反向射频传感系统成功地应用于实际样品中 Tyr 的定量分析，以及与伪彩色辅助方法相结合后发酵食品样品中 Tyr 的半定量视觉检测。

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Rational integration of fluorogenic and quenching activity of dopamine for reversed ratiometric and semi-quantitative visual detection of tyramine

A facile and efficient reversed ratiometric fluorescence (RF) method has been developed for the sensitive and accurate detection of tyramine (Tyr), an essential marker for food spoilage in fermented or aged foods. This method utilizes an “activation-quenching integration” strategy involving the fluorogenic reactivity and quenching capacity of dopamine (DA), the tyrosinase (TYR) catalyzed products of Tyr. Under alkaline conditions, DA reacts with resorcinol to form azamonardine, exhibiting strong blue emission. Additionally, DA is oxidized to o-dopaquinone by TYR, which quenches the red fluorescence of CdTe quantum dots (QDs). Under optimized conditions, a Tyr-specific RF sensor was established, featuring a linear detection range from 0.1 to 6 mg/mL and a detection limit of 8.50 × 10⁻³ mg/L. The fabricated reversed RF sensing system was successfully applied for quantitative analysis of Tyr in real samples and semi-quantitative visual detection of Tyr in fermented food samples upon integration with a pseudo-color-assisted approach.

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来源期刊

Dyes and Pigments 工程技术-材料科学：纺织

CiteScore

8.20

自引率

13.30%

发文量

933

审稿时长

33 days

期刊介绍： Dyes and Pigments covers the scientific and technical aspects of the chemistry and physics of dyes, pigments and their intermediates. Emphasis is placed on the properties of the colouring matters themselves rather than on their applications or the system in which they may be applied. Thus the journal accepts research and review papers on the synthesis of dyes, pigments and intermediates, their physical or chemical properties, e.g. spectroscopic, surface, solution or solid state characteristics, the physical aspects of their preparation, e.g. precipitation, nucleation and growth, crystal formation, liquid crystalline characteristics, their photochemical, ecological or biological properties and the relationship between colour and chemical constitution. However, papers are considered which deal with the more fundamental aspects of colourant application and of the interactions of colourants with substrates or media. The journal will interest a wide variety of workers in a range of disciplines whose work involves dyes, pigments and their intermediates, and provides a platform for investigators with common interests but diverse fields of activity such as cosmetics, reprographics, dye and pigment synthesis, medical research, polymers, etc.