Mahmoud AbouLaila , Maram Mahmoud , Heba Wheeb , Makoto Igarashi , Ahmed Elkhtam , Soad Menshawy
{"title":"埃及米努菲亚省鸡中五倍子蛔虫的流行情况和分子特征","authors":"Mahmoud AbouLaila , Maram Mahmoud , Heba Wheeb , Makoto Igarashi , Ahmed Elkhtam , Soad Menshawy","doi":"10.1016/j.vprsr.2024.101170","DOIUrl":null,"url":null,"abstract":"<div><div><em>Ascaridia galli</em> causes weight loss, emaciation, anemia, decreased egg production, and sometimes, intestinal obstruction and death in birds, leading to economic losses in the poultry industry. This research aimed to record the occurrence of <em>A. galli</em> in free-range chickens at farmers' houses in three villages in Ashmoun City, Minoufiya Governorate, Egypt. Additionally, molecular characterization was conducted using internal transcribed spacer 1 (ITS-1) region PCR and sequence analysis. A total of 570 chickens from 570 small flocks, each ranging in size from 10 to 20 free-range chickens, were investigated for the presence of <em>A. galli</em>. DNA was extracted from 10 adult worms collected from Shoshai and Elmanil-Dowib villages and subjected to PCR amplification and sequence analysis. The overall occurrence of <em>A. galli</em> in Ashmoun City was 38 %, with Elmanil-Dowib village having the highest prevalence at 45 %. The oldest chickens, over one year old, had the highest occurrence rate at 56 %, while females showed a higher occurrence (44 %) than males (15 %). The highest occurrence was observed during winter (47 %). The mean intensity and abundance of <em>A. galli</em> in Ashmoun were 5.52 and 2.07, respectively. The ITS-1 sequences of <em>A. galli</em> from Ashmoun, Minoufiya, Egypt had up to 100 % identity and clustered within the same genetic clade as <em>A. galli</em> from Egypt, Poland, and Bangladesh. Haplotype and nucleotide diversity analyses revealed that the sequences from Ashmoun represent a single haplotype and show no nucleotide mutations. This study presents the first report of the occurrence and genetic characterization of <em>A. galli</em> in Baladi chickens in Ashmoun City, Minoufiya, Egypt, thereby enhancing the understanding of <em>A. galli</em> epidemiology in this district and developing effective control strategies.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"57 ","pages":"Article 101170"},"PeriodicalIF":1.4000,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence and molecular characterization of Ascaridia galli in chickens from Minoufiya Governorate, Egypt\",\"authors\":\"Mahmoud AbouLaila , Maram Mahmoud , Heba Wheeb , Makoto Igarashi , Ahmed Elkhtam , Soad Menshawy\",\"doi\":\"10.1016/j.vprsr.2024.101170\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>Ascaridia galli</em> causes weight loss, emaciation, anemia, decreased egg production, and sometimes, intestinal obstruction and death in birds, leading to economic losses in the poultry industry. This research aimed to record the occurrence of <em>A. galli</em> in free-range chickens at farmers' houses in three villages in Ashmoun City, Minoufiya Governorate, Egypt. Additionally, molecular characterization was conducted using internal transcribed spacer 1 (ITS-1) region PCR and sequence analysis. A total of 570 chickens from 570 small flocks, each ranging in size from 10 to 20 free-range chickens, were investigated for the presence of <em>A. galli</em>. DNA was extracted from 10 adult worms collected from Shoshai and Elmanil-Dowib villages and subjected to PCR amplification and sequence analysis. The overall occurrence of <em>A. galli</em> in Ashmoun City was 38 %, with Elmanil-Dowib village having the highest prevalence at 45 %. The oldest chickens, over one year old, had the highest occurrence rate at 56 %, while females showed a higher occurrence (44 %) than males (15 %). The highest occurrence was observed during winter (47 %). The mean intensity and abundance of <em>A. galli</em> in Ashmoun were 5.52 and 2.07, respectively. The ITS-1 sequences of <em>A. galli</em> from Ashmoun, Minoufiya, Egypt had up to 100 % identity and clustered within the same genetic clade as <em>A. galli</em> from Egypt, Poland, and Bangladesh. Haplotype and nucleotide diversity analyses revealed that the sequences from Ashmoun represent a single haplotype and show no nucleotide mutations. This study presents the first report of the occurrence and genetic characterization of <em>A. galli</em> in Baladi chickens in Ashmoun City, Minoufiya, Egypt, thereby enhancing the understanding of <em>A. galli</em> epidemiology in this district and developing effective control strategies.</div></div>\",\"PeriodicalId\":23600,\"journal\":{\"name\":\"Veterinary parasitology, regional studies and reports\",\"volume\":\"57 \",\"pages\":\"Article 101170\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2024-11-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary parasitology, regional studies and reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405939024002065\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology, regional studies and reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405939024002065","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Prevalence and molecular characterization of Ascaridia galli in chickens from Minoufiya Governorate, Egypt
Ascaridia galli causes weight loss, emaciation, anemia, decreased egg production, and sometimes, intestinal obstruction and death in birds, leading to economic losses in the poultry industry. This research aimed to record the occurrence of A. galli in free-range chickens at farmers' houses in three villages in Ashmoun City, Minoufiya Governorate, Egypt. Additionally, molecular characterization was conducted using internal transcribed spacer 1 (ITS-1) region PCR and sequence analysis. A total of 570 chickens from 570 small flocks, each ranging in size from 10 to 20 free-range chickens, were investigated for the presence of A. galli. DNA was extracted from 10 adult worms collected from Shoshai and Elmanil-Dowib villages and subjected to PCR amplification and sequence analysis. The overall occurrence of A. galli in Ashmoun City was 38 %, with Elmanil-Dowib village having the highest prevalence at 45 %. The oldest chickens, over one year old, had the highest occurrence rate at 56 %, while females showed a higher occurrence (44 %) than males (15 %). The highest occurrence was observed during winter (47 %). The mean intensity and abundance of A. galli in Ashmoun were 5.52 and 2.07, respectively. The ITS-1 sequences of A. galli from Ashmoun, Minoufiya, Egypt had up to 100 % identity and clustered within the same genetic clade as A. galli from Egypt, Poland, and Bangladesh. Haplotype and nucleotide diversity analyses revealed that the sequences from Ashmoun represent a single haplotype and show no nucleotide mutations. This study presents the first report of the occurrence and genetic characterization of A. galli in Baladi chickens in Ashmoun City, Minoufiya, Egypt, thereby enhancing the understanding of A. galli epidemiology in this district and developing effective control strategies.
期刊介绍:
Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).