从土耳其自然感染的狗身上检测和分离出婴儿利什曼原虫

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2024-11-12 DOI:10.1016/j.vprsr.2024.101154

Aysegul Damlapinar , Umut Berberoglu , Mesut Mungan , Bekir Celebi , Aycan N. Gazyagci , Kader Yildiz

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引用次数: 0

摘要

一只生活在土耳其基里凯莱省（Kirikkale）农村地区的一岁大杂交雄犬被主人带到兽医诊所就诊，它出现了一些临床症状，如恶病质、脱发、剥脱性皮炎、脱毛和淋巴结肿大。该犬出现贫血、白细胞减少、高球蛋白血症和胆红素血症。使用利什曼病 IgG/IgM 快速检测试剂盒检测出血清阳性。在淋巴结抽吸物中观察到寄生虫的变形体。在从 Novy-MacNeal-Nicolle 培养基（NNN）试管中获得的样本中观察到了存活的原生体。根据生产商的说明，使用 DNA 提取试剂盒从 NNN 培养基中产生的原生体中提取寄生虫 DNA。结果显示，HSP20 和 HSP70 分别有 350 bp 和 730 bp 的特异性条带。此外，BLASTn 分析表明，这些序列与 GenBank 中的婴儿痢疾杆菌序列相似度为 100%。这些序列已提交至 GenBank（登录号：OR806945 和 OR806946）。据作者所知，这是存放在土耳其安卡拉卫生部公共卫生总局微生物参考实验室和生物产品部的第一个狗分离物。该分离物（TR_L. infantum CanL-1_damla）已在-150 °C下低温保存，以备进一步研究。

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Detection and isolation of Leishmania infantum from natural infected dog in Türkiye

Some clinical signs such as cachexia, alopecia, exfoliative dermatitis, hair loss, and swollen lymph nodes were observed in a one-year-old crossbred male dog living in a rural area of Kirikkale province, Türkiye, was presented to the veterinary clinic by its owner. Anaemia, leucopenia, hyperglobulinemia, and bilirubinemia were detected. Seropositivity was detected using Leishmania IgG/IgM Rapid Test. The amastigote forms of the parasite were observed in the lymph node aspirates. Viable promastigotes were observed in the samples obtained from the Novy-MacNeal-Nicolle medium (NNN) tubes. The parasite DNA was extracted from the promastigotes produced in the NNN medium using a DNA extraction kit according to the manufacturer's instructions. The specific bands indicating the gene regions of L. infantum (350 bp and 730 bp for HSP20 and HSP70, respectively) were observed. Additionally, BLASTn analysis revealed 100 % similarity with GenBank-deposited L. infantum sequences. The sequences were submitted to GenBank (Accession numbers: OR806945 and OR806946). According to the author's knowledge, it is the first dog isolate deposited in the collection of the Microbiology Reference Laboratories and Biological Products Department, General Directorate of Public Health, Ministry of Health, Ankara, Türkiye. The isolate (TR_L. infantum CanL-1_damla) has been preserved by cryopreservation at −150 °C for further studies.

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).