新型细菌素 PCM7-4 的鉴定和表征及其对李斯特菌的抗菌活性

IF 6.1 1区 生物学 Q1 MICROBIOLOGY
Haotian Ma , Yuexia Ding , Jinju Peng , Yang Li , Ruixue Pan , Yuner Long , Yining Zhao , Rongxian Guo , Yi Ma
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引用次数: 0

摘要

单核细胞增生李斯特菌是一种导致人畜共患病的致病菌,由于耐药菌的不断出现,迫切需要寻找新型的抗单核细胞增生李斯特菌药物。在这项研究中,我们通过 16S rRNA 序列分析,从海水中分离并鉴定出了一株产细菌素的 CM7-4 菌株,它就是 Velezensis 杆菌。此外,我们还成功地从枯草芽孢杆菌 CM7-4 中纯化出一种名为 PCM7-4 的新型细菌素。经测定,PCM7-4 的分子量为 40,228.99 Da。值得注意的是,PCM7-4 对革兰氏阳性菌和革兰氏阴性菌都具有广谱抗菌活性,特别是对李斯特菌的最低抑菌浓度(MIC)为 5.625 μg/mL。它在 pH 值为 2-12 的范围内具有耐热性和高稳定性,同时对蛋白酶 K 降解敏感,且未观察到任何溶血活性。此外,扫描电镜分析表明,PCM7-4 能有效抑制李斯特菌细胞中生物膜的形成并破坏细胞膜。转录组分析表明,PCM7-4 对与关键代谢途径相关的基因产生了影响,包括次生代谢物的生物合成、磷酸转移酶系统(PTS)和淀粉/蔗糖代谢。这些发现凸显了细菌素 PCM7-4 在开发针对食源性致病菌的有效抗菌干预措施方面的巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and characterization of a novel bacteriocin PCM7-4 and its antimicrobial activity against Listeria monocytogenes
Listeria monocytogenes, a pathogenic bacterium causing zoonotic diseases, necessitates the urgent search for novel anti-Listeria monocytogenes drugs due to the continuous emergence of drug-resistant bacteria. In this study, we isolated and identified a bacteriocin-producing strain CM7–4 from seawater as Bacillus velezensis through 16S rRNA sequence analysis. Moreover, we successfully purified a novel bacteriocin named PCM7–4 from Bacillus velezensis CM7–4. The molecular weight of PCM7–4 was determined to be 40,228.99 Da. Notably, PCM7–4 exhibited broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria with a minimum inhibitory concentration (MIC) of 5.625 μg/mL against Listeria monocytogenes specifically. It demonstrated heat resistance and high stability within the pH range of 2–12 while being sensitive to proteinase K degradation without any observed hemolytic activity. Furthermore, SEM analysis revealed that PCM7–4 effectively inhibited biofilm formation and disrupted cell membranes in Listeria monocytogenes cells. Transcriptome analysis revealed that PCM7–4 exerts an impact on genes associated with crucial metabolic pathways, encompassing the biosynthesis of secondary metabolites, phosphotransferase systems (PTS), and starch/sucrose metabolism. These findings highlight the significant potential of bacteriocin PCM7–4 for the development of effective antimicrobial interventions targeting food-borne pathogenic bacteria.
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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