利用两阶段培养系统和生物质增值技术协同微藻共培养处理城市污水

IF 4.6 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Algal Research-Biomass Biofuels and Bioproducts Pub Date : 2024-11-15 DOI:10.1016/j.algal.2024.103800

Rahul Kumar Goswami , Sanjeet Mehariya , Pradeep Verma

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引用次数: 0

摘要

本研究旨在开发不同的微藻类协同培养物 Tetraselmis indica BDUG001 (TS) 和 Picochlorum sp. (PC)，用于处理和循环利用未经处理的城市污水（MWW）。在去除营养物质和生产生物大分子方面，采用了 75 % 原城市污水 + 25 % ASN-III、50 % 二级处理城市污水（STMW） + 50 % ASN-III、50 % TS 处理城市污水（TSTMWW） + 50 % ASN-III。其中，2TS:1PC 和 2PC:1TS共培养在 75 % 原 MWW + 25 % ASN-III 和 50 % TSTMWW + 50 % ASN-III 中分别表现出最高的养分去除效率（NRE）和生物质及生物大分子产量。根据上述结果，开发了两阶段培养系统（TSCS），以利用微立式玻璃光生物反应器（MVG-PBR）最大限度地提高萌发率和生物大分子产量。第一和第二阶段培养采用 2TS:1PC 和 2PC:1TS 的共培养比例，分别记为 2TS:1PC (S1) 和 2PC:TS (S2)。将联合培养比例与单一培养的 TS（S1）和 PC（S2）进行了比较，并进行了成本分析，以评估该工艺的可行性。结果表明，在 TSCS 的第一阶段，2TS:1PC（S1）和 TS（S1）共培养可实现 COD（95 % 和 91.42 %）、TN（93.06 % 和 89.54 %）和 TP（100 % 和 93.87 %），并分别产生了干生物量（3.11 和 2.75 克/升）、脂质含量（46 和 42.17 %）、FAME 含量（82.25 和 32.01 %）、虾青素（1.12 和 0.93 毫克/克 DCW）和β-胡萝卜素（7.01 和 3.30 毫克/克 DCW）。在 TSCS 的第二阶段，2PC:1TS（S2）和 PC（S2）共培养表现出 COD（95.50 % 和 93.28 %）、TN（98.97 % 和 94.38 %）和 TP（100 % 和 94.50 %）的凈去除率，并产生生物量（3.在生物量（3.24 和 2.78 克/升）、脂质含量（48.50 和 40.70%）、FAME 含量（86.78 和 44.20%）、虾青素（1.15 和 0.71 毫克/克 DCW）和 β-胡萝卜素（5.14 和 5.33 毫克/克 DCW）方面分别取得了显著成效。成本分析表明，与单一养殖的 TSSCS 相比，协同养殖辅助 TSSCS 的收益更高。

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Synergistic microalgal co-cultivation for treatment of municipal wastewater using a two-stage cultivation system and biomass valorization

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Synergistic microalgal co-cultivation for treatment of municipal wastewater using a two-stage cultivation system and biomass valorization

The present study aimed to develop different microalgal co-cultivation Tetraselmis indica BDUG001 (TS) and Picochlorum sp. (PC) for the treatment and recycling of raw municipal wastewater (MWW). The nutrient removal and biomolecules production, 75 % raw MWW + 25 % ASN-III, 50 % secondary treated municipal wastewater (STMWW) + 50 % ASN-III, and 50 % TS-treated MWW (TSTMWW) + 50 % ASN-III was used. Among them, co-cultivation 2TS:1PC and 2PC:1TS exhibited maximum nutrient removal efficiency (NRE) and biomass and biomolecules production in 75 % raw MWW + 25 % ASN-III, and 50 % TSTMWW + 50 % ASN-III, respectively. Based on the above outcomes, two-stage cultivation systems (TSCSs) were developed to maximize the NRE and biomolecules production using the micro vertical glass photobioreactor (MVG-PBR). 2TS:1PC and 2PC:1TS co-cultivation ratio was used for first and second stage of cultivation and noted as 2TS:1PC (S1) and 2PC:TS (S2), respectively. The co-cultivation ratios were compared with monoculture TS (S1) and PC (S2), and the cost analysis was performed to assess the viability of the process. The obtained results showed that during the first stage of TSCSs, co-cultivation 2TS:1PC (S1) and TS (S1) exhibited NRE of COD (95 and 91.42 %), TN (93.06 and 89.54 %) and TP (100 and 93.87 %), and generated dry biomass (3.11 and 2.75 g/L), lipid content (46 and 42.17 %) having FAME content (82.25 and 32.01 %), astaxanthin (1.12 and 0.93 mg/g DCW) and β-carotene (7.01 and 3.30 mg/g DCW), respectively. During the second stage of TSCSs, co-cultivation 2PC:1TS (S2) and PC (S2) exhibited NRE of COD (95.50 and 93.28 %), TN (98.97 and 94.38 %) and TP (100 and 94.50 %), and generated biomass (3.24 and 2.78 g/L), lipid content (48.50 and 40.70 %) having FAME content (86.78 and 44.20 %), astaxanthin (1.15 and 0.71 mg/g DCW) and β-carotene (5.14 and 5.33 mg/g DCW), respectively. The cost analysis suggested that co-cultivation-assisted TSSCSs generated more revenue compared to monoculture-based TSCSs.

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来源期刊

Algal Research-Biomass Biofuels and Bioproducts BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

9.40

自引率

7.80%

发文量

332

期刊介绍： Algal Research is an international phycology journal covering all areas of emerging technologies in algae biology, biomass production, cultivation, harvesting, extraction, bioproducts, biorefinery, engineering, and econometrics. Algae is defined to include cyanobacteria, microalgae, and protists and symbionts of interest in biotechnology. The journal publishes original research and reviews for the following scope: algal biology, including but not exclusive to: phylogeny, biodiversity, molecular traits, metabolic regulation, and genetic engineering, algal cultivation, e.g. phototrophic systems, heterotrophic systems, and mixotrophic systems, algal harvesting and extraction systems, biotechnology to convert algal biomass and components into biofuels and bioproducts, e.g., nutraceuticals, pharmaceuticals, animal feed, plastics, etc. algal products and their economic assessment