Lin Chen , Qing Liu , Hailun Du , Jixiao Cui , Yuanquan Chen
{"title":"有机材料的回归通过改变反硝化微生物群落的组成而非数量,抑制了土壤中一氧化二氮的排放","authors":"Lin Chen , Qing Liu , Hailun Du , Jixiao Cui , Yuanquan Chen","doi":"10.1016/j.apsoil.2024.105759","DOIUrl":null,"url":null,"abstract":"<div><div>Organic materials returned to the field have a significant effect on N<sub>2</sub>O emissions from agricultural fields, but the knowledge about the relationship between soil denitrifying microorganisms and N<sub>2</sub>O emissions is limited. Hence, we delved deeper into the significance of denitrifying microorganisms in N2O emissions by examining the soil N2O emissions, gene copy numbers, and community structures of denitrifying microorganisms during the wheat harvest season, three years after the partial substitution of chemical nitrogen fertilizers with various organic materials, including straw, pig manure, and biogas residues. The results showed that compared with chemical fertilizer, straw return did not change N<sub>2</sub>O emission, and pig manure and biogas residue, especially pig manure return, significantly reduced N<sub>2</sub>O emission (62 % and 45 %). Organic materials return did not change the gene copy number of denitrifying microorganisms, but had a significant effect on the community structure. The relative abundance of genera in the three organic materials treatments differed significantly from the chemical fertilizer treatment. The pig manure treatment had marker genera in the <em>nosZ</em> gene. Among the <em>nirK</em>, <em>nirS</em>, and <em>nosZ</em> genes, <em>Sinorhizobium</em>, <em>norank_p_environment_samples</em>, and <em>unclassified_k_norank_d_bacteria</em>, respectively, had the greatest effect on N<sub>2</sub>O emissions. The results of the RDA and the minimum depth method indicated that K, pH, and SOC were the key environmental factors influencing the structural changes of <em>nirK</em>, <em>nirS</em> and <em>nosZ</em> communities. Overall, organic materials, especially pig manure, effectively suppressed N<sub>2</sub>O emissions by changing the relative abundance and community structure of the dominant genera of denitrifying microorganisms.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105759"},"PeriodicalIF":4.8000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Organic materials return suppressed soil N2O emissions by changing the composition instead of abundance of denitrifying microbial community\",\"authors\":\"Lin Chen , Qing Liu , Hailun Du , Jixiao Cui , Yuanquan Chen\",\"doi\":\"10.1016/j.apsoil.2024.105759\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Organic materials returned to the field have a significant effect on N<sub>2</sub>O emissions from agricultural fields, but the knowledge about the relationship between soil denitrifying microorganisms and N<sub>2</sub>O emissions is limited. Hence, we delved deeper into the significance of denitrifying microorganisms in N2O emissions by examining the soil N2O emissions, gene copy numbers, and community structures of denitrifying microorganisms during the wheat harvest season, three years after the partial substitution of chemical nitrogen fertilizers with various organic materials, including straw, pig manure, and biogas residues. The results showed that compared with chemical fertilizer, straw return did not change N<sub>2</sub>O emission, and pig manure and biogas residue, especially pig manure return, significantly reduced N<sub>2</sub>O emission (62 % and 45 %). Organic materials return did not change the gene copy number of denitrifying microorganisms, but had a significant effect on the community structure. The relative abundance of genera in the three organic materials treatments differed significantly from the chemical fertilizer treatment. The pig manure treatment had marker genera in the <em>nosZ</em> gene. Among the <em>nirK</em>, <em>nirS</em>, and <em>nosZ</em> genes, <em>Sinorhizobium</em>, <em>norank_p_environment_samples</em>, and <em>unclassified_k_norank_d_bacteria</em>, respectively, had the greatest effect on N<sub>2</sub>O emissions. The results of the RDA and the minimum depth method indicated that K, pH, and SOC were the key environmental factors influencing the structural changes of <em>nirK</em>, <em>nirS</em> and <em>nosZ</em> communities. Overall, organic materials, especially pig manure, effectively suppressed N<sub>2</sub>O emissions by changing the relative abundance and community structure of the dominant genera of denitrifying microorganisms.</div></div>\",\"PeriodicalId\":8099,\"journal\":{\"name\":\"Applied Soil Ecology\",\"volume\":\"204 \",\"pages\":\"Article 105759\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-11-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Soil Ecology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0929139324004906\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SOIL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Soil Ecology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0929139324004906","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}
Organic materials return suppressed soil N2O emissions by changing the composition instead of abundance of denitrifying microbial community
Organic materials returned to the field have a significant effect on N2O emissions from agricultural fields, but the knowledge about the relationship between soil denitrifying microorganisms and N2O emissions is limited. Hence, we delved deeper into the significance of denitrifying microorganisms in N2O emissions by examining the soil N2O emissions, gene copy numbers, and community structures of denitrifying microorganisms during the wheat harvest season, three years after the partial substitution of chemical nitrogen fertilizers with various organic materials, including straw, pig manure, and biogas residues. The results showed that compared with chemical fertilizer, straw return did not change N2O emission, and pig manure and biogas residue, especially pig manure return, significantly reduced N2O emission (62 % and 45 %). Organic materials return did not change the gene copy number of denitrifying microorganisms, but had a significant effect on the community structure. The relative abundance of genera in the three organic materials treatments differed significantly from the chemical fertilizer treatment. The pig manure treatment had marker genera in the nosZ gene. Among the nirK, nirS, and nosZ genes, Sinorhizobium, norank_p_environment_samples, and unclassified_k_norank_d_bacteria, respectively, had the greatest effect on N2O emissions. The results of the RDA and the minimum depth method indicated that K, pH, and SOC were the key environmental factors influencing the structural changes of nirK, nirS and nosZ communities. Overall, organic materials, especially pig manure, effectively suppressed N2O emissions by changing the relative abundance and community structure of the dominant genera of denitrifying microorganisms.
期刊介绍:
Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.