13C 标记的玉米秸秆分解过程中的土壤孔隙结构塑造了土壤微生物群落的组成和结构

IF 4.8 2区 农林科学 Q1 SOIL SCIENCE
Tianyu Ding , Rui Qian , Zichun Guo , Xianjin Huang , Xinhua Peng
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引用次数: 0

摘要

土壤孔隙结构可调节水分流动、气体交换并决定养分的异质分布,从而对微生物产生重大影响。然而,在秸秆分解过程中,不同大小的孔隙对土壤微生物群落的具体贡献和限制,以及在整个过程中孔隙结构的变化仍是未知数。为了解决这个问题,我们使用 13C 标记的玉米秸秆与两种土壤类型进行了为期 57 天的土壤培养实验:沙江黑土(Vertisols)和Fluvo-aquic 土壤(Cambisols)。实验设置了两种容重:1.2 g cm-3 和 1.5 g cm-3。利用 X 射线微计算机断层扫描(μCT)对培养实验前后孔隙结构的变化进行了量化。为了分析秸秆中微生物 C 的含量,使用了 13C 磷脂脂肪酸(13C-PLFAs),并采用 16S rDNA 高通量测序来确定培养后的细菌群落结构。结果表明,在容重为 1.5 g cm-3 的条件下,沙江黑土的秸秆总磷脂-C 含量比氟水土增加了 50.5%(P < 0.05)。具体来说,革兰氏阳性菌(G+)、革兰氏阴性菌(G-)和放线菌的 PLFA-C 含量分别增加了 105.2 %、55.0 % 和 73.6 %(P < 0.05)。冗余分析(RDA)显示,直径(Ø)为 100 μm 的孔有利于微生物(G+、G- 和放线菌)的定殖。具体来说,细菌(包括 G+、G-和普通细菌,分别占秸秆富集的 PLFA-C 总量的 37.6%、15.7% 和 22.1%)是秸秆分解的关键因素,与直径为 50-200 μm 的孔的形成有关。与此同时,真菌活动是导致 20-50 μm 直径孔隙结构变化的主要原因。这些发现强调了 100 μm 直径孔隙在秸秆分解过程中为微生物群落创造了有利的物理环境,突出了微生物活动在形成土壤孔隙结构中的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Soil pore structure shaped compositions and structures of soil microbial community during 13C-labelled maize straw decomposition

Soil pore structure shaped compositions and structures of soil microbial community during 13C-labelled maize straw decomposition
Soil pore structure regulates water movement, gas exchange and determines the heterogeneous distribution of nutrients, thereby exerting significant influence on microorganisms. However, the specific contributions and limitations of pores of different sizes on soil microbial communities during straw decomposition, as well as the variations in pore structure throughout this process, remain unknown. To address this, a 57-day soil incubation experiment was conducted, using 13C-labelled maize straw with two soil types: Shajiang black soil (Vertisols) and Fluvo-aquic soil (Cambisols). The experiment was set at two bulk densities: 1.2 g cm−3 and 1.5 g cm−3. The variations in pore structure before and after the incubation experiment were quantified using X-ray micro-computed tomography (μCT). To analyze microbial C content from straw, 13C-Phospholipid fatty acids (13C-PLFAs) were used, and 16S rDNA high-throughput sequencing was employed to determine bacterial community structure after the incubation. The resulted showed that Shajiang black soil exhibited a 50.5 % increase of total PLFA-C content from straw than Fluvo-aquic soil at a bulk density of 1.5 g cm−3 (P < 0.05). Specifically, Gram-positive bacteria (G+), Gram-negative bacteria (G−) and actinobacteria PLFA-C content increased by 105.2 %, 55.0 % and 73.6 % (P < 0.05). Redundancy analysis (RDA) showed that >100 μm pores in diameter (Ø) facilitated the colonization of microorganisms (G+, G− and actinobacteria). Specifically, bacteria (including G+, G−, and common bacteria, accounted for 37.6 %, 15.7 % and 22.1 % of straw-derived PLFA-C in relation to the total amount of straw-enriched PLFA-C) were key contributors to straw decomposition and were associated with the formation of 50–200 μm Ø pores. Meanwhile, fungal activity was primarily responsible for changes in the 20–50 μm Ø pore structure. These findings emphasize that >100 μm Ø pores created a favorable physical environment for microbial communities on straw decomposition, highlighting the pivotal role of microbial activity in shaping soil pore structure.
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来源期刊
Applied Soil Ecology
Applied Soil Ecology 农林科学-土壤科学
CiteScore
9.70
自引率
4.20%
发文量
363
审稿时长
5.3 months
期刊介绍: Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.
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