细胞培养基中 L-谷氨酰胺的分解和氨的积累可抑制流感病毒的感染性。

IF 2.8 3区医学 Q3 VIROLOGY

Virology Pub Date : 2024-11-20 DOI:10.1016/j.virol.2024.110314

Nathanael B. Kegel , Andreas Kaufmann , Mikhail Matrosovich , Stefan Bauer , Jens Dorna

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引用次数: 0

摘要

铵盐和氯喹等阳离子溶酶体分子通过抵消内体酸化和阻碍病毒-内体融合，抑制甲型流感病毒（IAV）在细胞培养中的感染。在此，我们研究了添加了 L-谷氨酰胺的细胞培养基的储存对铵盐积累和 IAV 感染抑制的影响。在 DMEM 和 OptiMEM 培养基中观察到了与贮存有关的抑制作用，而在 RPMI 培养基中则没有观察到。我们的研究结果突显了考虑培养基中可能存在的病毒抑制溶酶体物质和/或其产生对流感病毒感染产生负面影响的重要性。

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Decomposition of L-glutamine and accumulation of ammonium in cell culture media inhibit infectivity of influenza viruses

Cationic lysosomotropic molecules such as ammonium salts and chloroquine inhibit influenza A virus (IAV) infection in cell culture by counteracting endosomal acidification and hampering viral-endosomal fusion. Here, we studied the effects of storage of L-glutamine-supplemented cell culture media on accumulation of ammonium and inhibition of IAV infection. The storage-related inhibitory effect was observed for DMEM and OptiMEM media but not for RPMI medium, and was more pronounced for IAV with pH-stable hemagglutinin. Our results highlight the importance to consider potential presence of virus-inhibiting lysosomotropic agents and/or their production in culture media with negative effect on influenza virus infection.

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来源期刊

Virology 医学-病毒学

CiteScore

6.00

自引率

0.00%

发文量

157

审稿时长

50 days

期刊介绍： Launched in 1955, Virology is a broad and inclusive journal that welcomes submissions on all aspects of virology including plant, animal, microbial and human viruses. The journal publishes basic research as well as pre-clinical and clinical studies of vaccines, anti-viral drugs and their development, anti-viral therapies, and computational studies of virus infections. Any submission that is of broad interest to the community of virologists/vaccinologists and reporting scientifically accurate and valuable research will be considered for publication, including negative findings and multidisciplinary work.Virology is open to reviews, research manuscripts, short communication, registered reports as well as follow-up manuscripts.