运动对改善与产妇超重和肥胖有关的胎盘不良环境的表观遗传效应。

IF 3 2区医学 Q2 DEVELOPMENTAL BIOLOGY

Placenta Pub Date : 2024-10-23 DOI:10.1016/j.placenta.2024.10.018

Chen Wang, Minghui Liu, Jie Yan, Jie Ning, Shuxian Wang, Huixia Yang

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引用次数: 0

摘要

目的我们旨在探讨孕产妇运动对改善与孕产妇超重/肥胖相关的胎盘不良环境是否具有表观遗传学效应：方法：基于从RCT队列中收集的样本，使用Infinium HumanMethylation850 BeadChip阵列和基于RNA测序的lncRNA分析，在16名参与或未参与运动干预的超重和肥胖妇女的胎盘中确定了不同的甲基化基因和表达的蛋白编码RNA。通过整合这些信息，进一步确定了潜在的靶基因。然后，对靶基因的甲基化和表达水平进行验证，并与胎盘氧化应激标志物和参与者的临床代谢参数进行相关性分析：结果：共检测到 3608 个具有显著差异的甲基化探针。运动组胎盘中 CBR1 基因在 CPG 位点启动子区域的甲基化水平显著降低，CBR1 基因表达水平显著升高。CBR1 基因表达水平与 DNA 甲基化水平呈负相关。此外，CBR1 基因表达水平与胎盘和脐带血样本中的 MDA 水平以及妊娠晚期的胰岛素抵抗水平呈负相关。此外，CBR1 甲基化水平与妊娠晚期空腹血浆葡萄糖和胰岛素抵抗水平呈正相关：DNA甲基化参与了运动对与母体超重/肥胖相关的不利胎盘环境的改善作用。

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Epigenetic effects of exercise on improving the adverse placental environment associated with maternal overweight and obesity

Objective

We aimed to explore whether maternal exercise has epigenetic effects on improving the adverse placental environment associated with maternal overweight/obesity.

Methods

Based on samples collected from an RCT cohort, differentially methylated genes and expressed protein-coding RNA were identified in the placentas of 16 women with overweight and obesity who did or did not participate in an exercise intervention using the Infinium HumanMethylation850 BeadChip array and RNA Sequencing-Based lncRNA Profiling. Potential target genes were further identified by integrating this information. Then, the target genes' methylation and expression levels were verified, and correlation analysis was performed with placental oxidative stress markers and participants’ clinical metabolic parameters.

Results

A total of 3608 significant differentially methylated probes were detected. The CBR1 gene, which was previously identified as a possible antioxidant, was significantly hypomethylated at CPG site promoter regions in placentas from the exercise group, and CBR1 gene expression levels were significantly higher. CBR1 gene expression levels were negatively associated with DNA methylation levels. Furthermore, CBR1 gene expression levels were negatively correlated with MDA levels in both placenta and cord blood samples and insulin resistance levels in late pregnancy. Additionally, CBR1 methylation levels were positively correlated with fasting plasma glucose and insulin resistance levels in late pregnancy.

Conclusion

DNA methylation is involved in the ameliorating effect of exercise on the adverse placental environment associated with maternal overweight/obesity.

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来源期刊

Placenta 医学-发育生物学

CiteScore

6.30

自引率

10.50%

发文量

391

审稿时长

78 days

期刊介绍： Placenta publishes high-quality original articles and invited topical reviews on all aspects of human and animal placentation, and the interactions between the mother, the placenta and fetal development. Topics covered include evolution, development, genetics and epigenetics, stem cells, metabolism, transport, immunology, pathology, pharmacology, cell and molecular biology, and developmental programming. The Editors welcome studies on implantation and the endometrium, comparative placentation, the uterine and umbilical circulations, the relationship between fetal and placental development, clinical aspects of altered placental development or function, the placental membranes, the influence of paternal factors on placental development or function, and the assessment of biomarkers of placental disorders.