外源硫化氢增效抗生素对多重耐药气单胞菌的再敏感作用

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Sahithya Selvakumar, Shubhi Singh, Priya Swaminathan
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引用次数: 0

摘要

抗生素耐药性(AMR)是由于抗生素的广泛过度使用而造成的日益严重的公共卫生威胁。具有抗药性的细菌可能从土壤或水中获得抗药性基因。细菌中内源性硫化氢(H2S)的产生使许多细菌具有抗生素耐受性,这表明细菌对抗生素具有普遍的防御机制。在这项研究中,我们分离并鉴定了从污染地区采集的土壤抗生素耐药细菌。经鉴定,一种抗生素耐药细菌不产生内源 H2S,因此我们可以研究外源 H2S 对其耐药机制的影响。因此,我们证明了通过将 H2S 与氨苄西林和庆大霉素等抗生素一起使用,可以逆转不同种类的抗生素耐药性。我们采用 Kirby-Bauer Disk-Diffusion、扫描电子显微镜和流式细胞仪分析等方法来评估 H2S 与氨苄西林和庆大霉素的抗菌活性。使用 DCFH-DA(ROS)测试评估了 H2S 的抗氧化效率,以及脂质过氧化和 LDH 活性。酶和非酶(SOD、CAT、GST 和 GSH)抗氧化研究进一步证实了这些结果。这些研究结果证明,H2S 是一种抗生素诱导剂,可导致细菌膜损伤、氧化应激以及 DNA 和蛋白质破坏。因此,提供外源 H2S 可以成为逆转抗生素耐药性的良药。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Resensitization of Multi Drug-Resistant Aeromonas caviae with Exogenous Hydrogen Sulfide Potentiated Antibiotics.

Antimicrobial resistance (AMR) is a growing public health threat caused by the widespread overuse of antibiotics. Bacteria with antibiotic resistance may acquire resistance genes from soil or water. Endogenous hydrogen sulfide (H2S) production in bacteria confers antibiotic tolerance in many, suggesting a universal defense mechanism against antibiotics. In this study, we isolated and identified soil-based antibiotic-resistant bacteria collected from contaminated areas. An antibiotic-resistant bacterium was identified as non-endogenous-H2S-producing, allowing us to examine the effect of exogenous H2S on its resistance mechanism. Therefore, we demonstrated that different classes of antibiotic resistance can be reverted by employing H2S with antibiotics like ampicillin and gentamicin. Methods like Kirby-Bauer Disk-Diffusion, Scanning Electron Microscopy, and Flow Cytometer analysis were performed to assess the antibacterial activity of H2S with ampicillin and gentamicin. The antioxidative efficiency of H2S was evaluated using the DCFH-DA (ROS) test, as well as lipid peroxidation, and LDH activity. These were further confirmed with enzymatic and non-enzymatic (SOD, CAT, GST, and GSH) antioxidant studies. These findings support H2S as an antibiotic-potentiator, causing bacterial membrane damage, oxidative stress, and disrupting DNA and proteins. Thus, supplying exogenous H2S can be a good agent for the reversal of Antibiotic resistance.

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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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