Yu Zhou , Jing Du , Yamei Liu , Jinfei Ma , Xiaofen Yuan , Jun Xia
{"title":"应用磁珠辅助液相色谱-串联质谱法分析血清胆汁酸谱:向自动质谱法迈进。","authors":"Yu Zhou , Jing Du , Yamei Liu , Jinfei Ma , Xiaofen Yuan , Jun Xia","doi":"10.1016/j.cca.2024.120058","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Magnetic bead-assisted extraction technology offers potential for automating mass spectrometry processes, yet research in this area remains limited. Here, we developed and validated a magnetic bead extraction method to simultaneously analyze 13 bile acid profiles in human serum.</div></div><div><h3>Methods</h3><div>A magnetic bead-assisted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for serum bile acid profiles. The linearity, accuracy, precision, matrix effect, carryover, stability test and interference test of the LC-MS/MS method were assessed. Comparative analysis involving 20 pregnant women evaluated different methodologies for diagnosing and monitoring intrahepatic cholestasis of pregnancy (ICP).</div></div><div><h3>Results</h3><div>The magnetic bead extraction method demonstrated analytical performance comparable to the traditional protein precipitation method. The method exhibited linear responses across the clinical reference interval for all 13 bile acids. Meanwhile, the recovery rates for bile acid analytes ranged from 85.14 to 114.43%, and the repeatability was within 1.68 to 10.83%, meeting acceptance criteria. Our magnetic bead-assisted extraction method showed no obvious matrix effects and carryover effects. The extracted specimens remained stable in the autosampler for 24 h, and the interference test suggested the bile acid profile results would not be significantly affected under different interfering substances. Furthermore, comparative studies with traditional protein precipitation method showed comparable results, and our method exhibited robust clinical consistency in monitoring the efficacy of ICP patients.</div></div><div><h3>Conclusions</h3><div>We developed a straightforward magnetic bead extraction method coupled with LC-MS/MS for bile acid profiles. It can be applied to the clinical routine diagnosis of ICP and facilitate the automated mass spectrometry processes.</div></div>","PeriodicalId":10205,"journal":{"name":"Clinica Chimica Acta","volume":"566 ","pages":"Article 120058"},"PeriodicalIF":3.2000,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application of magnetic bead-assisted liquid chromatography-tandem mass spectrometry method for analyzing serum bile acid profiles: Advancing toward automated mass spectrometry\",\"authors\":\"Yu Zhou , Jing Du , Yamei Liu , Jinfei Ma , Xiaofen Yuan , Jun Xia\",\"doi\":\"10.1016/j.cca.2024.120058\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Magnetic bead-assisted extraction technology offers potential for automating mass spectrometry processes, yet research in this area remains limited. Here, we developed and validated a magnetic bead extraction method to simultaneously analyze 13 bile acid profiles in human serum.</div></div><div><h3>Methods</h3><div>A magnetic bead-assisted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for serum bile acid profiles. The linearity, accuracy, precision, matrix effect, carryover, stability test and interference test of the LC-MS/MS method were assessed. Comparative analysis involving 20 pregnant women evaluated different methodologies for diagnosing and monitoring intrahepatic cholestasis of pregnancy (ICP).</div></div><div><h3>Results</h3><div>The magnetic bead extraction method demonstrated analytical performance comparable to the traditional protein precipitation method. The method exhibited linear responses across the clinical reference interval for all 13 bile acids. Meanwhile, the recovery rates for bile acid analytes ranged from 85.14 to 114.43%, and the repeatability was within 1.68 to 10.83%, meeting acceptance criteria. Our magnetic bead-assisted extraction method showed no obvious matrix effects and carryover effects. The extracted specimens remained stable in the autosampler for 24 h, and the interference test suggested the bile acid profile results would not be significantly affected under different interfering substances. Furthermore, comparative studies with traditional protein precipitation method showed comparable results, and our method exhibited robust clinical consistency in monitoring the efficacy of ICP patients.</div></div><div><h3>Conclusions</h3><div>We developed a straightforward magnetic bead extraction method coupled with LC-MS/MS for bile acid profiles. It can be applied to the clinical routine diagnosis of ICP and facilitate the automated mass spectrometry processes.</div></div>\",\"PeriodicalId\":10205,\"journal\":{\"name\":\"Clinica Chimica Acta\",\"volume\":\"566 \",\"pages\":\"Article 120058\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-11-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinica Chimica Acta\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0009898124023118\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinica Chimica Acta","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009898124023118","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Application of magnetic bead-assisted liquid chromatography-tandem mass spectrometry method for analyzing serum bile acid profiles: Advancing toward automated mass spectrometry
Background
Magnetic bead-assisted extraction technology offers potential for automating mass spectrometry processes, yet research in this area remains limited. Here, we developed and validated a magnetic bead extraction method to simultaneously analyze 13 bile acid profiles in human serum.
Methods
A magnetic bead-assisted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for serum bile acid profiles. The linearity, accuracy, precision, matrix effect, carryover, stability test and interference test of the LC-MS/MS method were assessed. Comparative analysis involving 20 pregnant women evaluated different methodologies for diagnosing and monitoring intrahepatic cholestasis of pregnancy (ICP).
Results
The magnetic bead extraction method demonstrated analytical performance comparable to the traditional protein precipitation method. The method exhibited linear responses across the clinical reference interval for all 13 bile acids. Meanwhile, the recovery rates for bile acid analytes ranged from 85.14 to 114.43%, and the repeatability was within 1.68 to 10.83%, meeting acceptance criteria. Our magnetic bead-assisted extraction method showed no obvious matrix effects and carryover effects. The extracted specimens remained stable in the autosampler for 24 h, and the interference test suggested the bile acid profile results would not be significantly affected under different interfering substances. Furthermore, comparative studies with traditional protein precipitation method showed comparable results, and our method exhibited robust clinical consistency in monitoring the efficacy of ICP patients.
Conclusions
We developed a straightforward magnetic bead extraction method coupled with LC-MS/MS for bile acid profiles. It can be applied to the clinical routine diagnosis of ICP and facilitate the automated mass spectrometry processes.
期刊介绍:
The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)
Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells.
The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.