低碳氮比条件下需氧反硝化马林杆菌(Marinobacter alkaliphilus SBY-1)的反硝化能力和氮代谢途径。

IF 8.2 1区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Science of the Total Environment Pub Date : 2024-12-20 Epub Date: 2024-11-24 DOI:10.1016/j.scitotenv.2024.177651
Chao Pan, Yang-Guo Zhao, Progress Mupindu, Shuxue Zhao
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引用次数: 0

摘要

海水养殖尾水的特点是碳/氮比低,因此由于碳源不足而阻碍了传统的异养反硝化过程。因此,迫切需要具有异养硝化和好氧反硝化(HN-AD)功能的低营养海洋细菌来对好氧生物滤池进行生物增殖。本研究分离了碱性海洋细菌 SBY-1,并证实其在初始硝酸盐浓度为 800 mg/L、pH 值为 7、盐度为 20‰、醋酸钠为碳源、低 C/N 比为 3.6 的条件下,具有最佳硝酸盐去除能力,去除率为 716 mg/L-d,且无氨产生或亚硝酸盐积累。SBY-1 还表现出异养硝化能力,以氨氮为氮源时,氨氮去除率最高可达 69.21%。参与 HN-AD 过程的酶包括氨单氧酶(AMO)、硝酸还原酶(NR)和亚硝酸还原酶(NIR),在 SBY-1 中均有检测到,其中 NR 和 NIR 的活性更强。此外,EPS 和自动聚集分析表明,SBY-1 在高进氮浓度条件下表现出卓越的自动聚集能力,使其更适合形成生物膜,并进一步应用于基于生物膜的脱硝过程。基因组分析发现了与 Nar、Nap、Nas、Nir、Nif、Nrt、Nrf、Nor、Nos 相关的基因,证实 SBY-1 具有完整的 HN-AD 氮代谢途径。预测的 SBY-1 氮代谢途径为 NO3--N → NO2--N → NO→N2O → N2。这些发现为 SBY-1 在较低 C/N 条件下高效去除硝酸盐提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The denitrification ability and nitrogen metabolism pathway of aerobic denitrifier Marinobacter alkaliphilus SBY-1 under low C/N ratios.

Mariculture tail water is characterized as the low C/N ratios and thus blocks the conventional heterotrophic denitrification process due to insufficient carbon source. Therefore, oligotrophic marine bacteria with heterotrophic nitrification and aerobic denitrification (HN-AD) are urgently required to bioaugment aerobic biological filter. In this study, Marinobacter alkaliphilus SBY-1 was isolated and confirmed optimal nitrate removal capacity at a rate of 716 mg/L·d without ammonia production or nitrite accumulation under initial nitrate concentration of 800 mg/L, pH 7, salinity 20 ‰, sodium acetate as the carbon source, and low C/N ratios of 3.6. SBY-1 also demonstrated heterotrophic nitrification capability with a maximum ammonia removal rate reaching 69.21 % when ammonia was used as the nitrogen source. The enzymes involved in the HN-AD process including ammonia monooxygenase (AMO), nitrate reductase (NR), and nitrite reductase (NIR) were all detected in SBY-1 with superior activity observed for NR and NIR. Additionally, analysis of EPS and auto-aggregation revealed that SBY-1 exhibited excellent auto-aggregation ability under high influent nitrogen concentration conditions, making it more suitable for biofilm formation and further application in biofilm-based denitrification process. Genome analysis identified genes associated with Nar, Nap, Nas, Nir, Nif, Nrt, Nrf, Nor, Nos which confirmed that SBY-1 possessed a complete HN-AD pathway for nitrogen metabolism. The predicted nitrogen metabolism pathway of SBY-1 was NO3--N → NO2--N → NO→N2O → N2. These findings provide new insights into the efficient removal of nitrate by SBY-1 under lower C/N conditions.

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来源期刊
Science of the Total Environment
Science of the Total Environment 环境科学-环境科学
CiteScore
17.60
自引率
10.20%
发文量
8726
审稿时长
2.4 months
期刊介绍: The Science of the Total Environment is an international journal dedicated to scientific research on the environment and its interaction with humanity. It covers a wide range of disciplines and seeks to publish innovative, hypothesis-driven, and impactful research that explores the entire environment, including the atmosphere, lithosphere, hydrosphere, biosphere, and anthroposphere. The journal's updated Aims & Scope emphasizes the importance of interdisciplinary environmental research with broad impact. Priority is given to studies that advance fundamental understanding and explore the interconnectedness of multiple environmental spheres. Field studies are preferred, while laboratory experiments must demonstrate significant methodological advancements or mechanistic insights with direct relevance to the environment.
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