缺乏 ancV1R 的雌性小鼠信息素检测能力受损,性行为异常。

IF 8.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hiro Kondo, Tetsuo Iwata, Koji Sato, Riseru Koshiishi, Hikoyu Suzuki, Ken Murata, Marc Spehr, Kazushige Touhara, Masato Nikaido, Junji Hirota
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引用次数: 0

摘要

古绒毛膜受体 1 型(ancV1R)是一种推定的绒毛膜受体,在多种脊椎动物中高度保守,在大多数绒毛膜感觉神经元中表达,与典型的绒毛膜受体 V1R 和 V2R 共同表达。ancV1R 的假基因化与绒毛器官(VNO)的退化密切相关,表明其在信息素感应中的关键作用。然而,ancV1R 的具体作用仍然未知。在本研究中,为了阐明ancV1R的功能,我们对ancV1R缺陷的雌性小鼠进行了表型分析。行为分析表明,ancV1R 缺失的雌性小鼠对雄性的性行为表现出排斥反应,对雄性的尿液没有偏好。生理学分析表明,ancV1R功能缺失突变降低了VNO对各种信息素线索的反应,包括雄性尿液、性增强信息素外分泌腺分泌肽1(ESP1)和β-雌二醇3-硫酸盐。预先暴露于ESP1并不能克服ancV1R缺乏引起的排斥行为。对绒毛神经系统神经活动的分析表明,突变雌性在与雄性接触时,内侧杏仁核和后内侧皮层杏仁核的反应增加,但对雄性尿液的反应却没有增加。此外,在与雄性接触时,ancV1R缺陷雌性的外侧隔膜(一个与应激相关的脑区)神经活动增加,同时应激激素水平升高。而只接触雄性尿液的雌性则没有观察到这种效应。这些研究结果表明,在雌性动物中,ancV1R能促进VNO对信息素刺激的反应,并在将雄性动物视为交配伙伴的过程中发挥关键作用。ancV1R 的缺失会导致雄性感知失灵,从而导致异常性行为和与雄性接触时的应激反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impaired pheromone detection and abnormal sexual behavior in female mice deficient for ancV1R.

Ancient vomeronasal receptor type-1 (ancV1R), a putative vomeronasal receptor, is highly conserved across a wide range of vertebrates and is expressed in the majority of vomeronasal sensory neurons, co-expressing with canonical vomeronasal receptors, V1Rs and V2Rs. The pseudogenization of ancV1R is closely associated with vomeronasal organ (VNO) degeneration, indicating its critical role in pheromone sensing. However, the specific role of ancV1R remains unknown. In this study, to elucidate the function of ancV1R, we conducted phenotypic analyses of ancV1R-deficient female mice. Behavioral analyses showed that ancV1R-deficient females exhibited rejective responses toward male sexual behavior and displayed no preference for male urine. Physiological analyses demonstrate that the loss-of-function mutation of ancV1R reduced VNO response to various pheromone cues, including male urine, the sexual enhancing pheromone exocrine gland-secreting peptide 1 (ESP1), and β-estradiol 3-sulfate. Pre-exposure to ESP1 did not overcome the rejection behavior caused by ancV1R deficiency. Analysis of neural activity in the vomeronasal system revealed increased responses in the medial amygdala and posteromedial cortical amygdala of mutant females upon contact with males but not in response to male urine alone. Additionally, upon male contacts, ancV1R-deficient females exhibited increased neural activity in the lateral septum, a stress-associated brain region, along with elevated stress hormone levels. Such effects were not observed in females exposed solely to male urine. These findings suggest that, in females, ancV1R facilitates VNO responses to pheromone stimuli and plays a crucial role in perceiving males as mating partners. The absence of ancV1R results in failure of male perception, leading to abnormal sexual behaviors and stress responses upon male contact.

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来源期刊
Current Biology
Current Biology 生物-生化与分子生物学
CiteScore
11.80
自引率
2.20%
发文量
869
审稿时长
46 days
期刊介绍: Current Biology is a comprehensive journal that showcases original research in various disciplines of biology. It provides a platform for scientists to disseminate their groundbreaking findings and promotes interdisciplinary communication. The journal publishes articles of general interest, encompassing diverse fields of biology. Moreover, it offers accessible editorial pieces that are specifically designed to enlighten non-specialist readers.
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