欧姆加热辅助糖化反应对大豆分离蛋白壳聚糖复合物性质的影响

IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yuting Wang, Ning Wang, Boyu Liu, Cuiping Han, Tong Wang, Mingzhe Pan, Dianyu Yu
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引用次数: 0

摘要

本研究旨在探讨传统加热和欧姆加热(OH)处理糖化反应的进展,以及不同电场强度对糖基化蛋白质的结构、理化和功能特性的影响。研究结果表明,在减少游离氨基和提高 420 纳米吸光度方面,欧姆加热处理比传统加热更有效。同时,大豆分离蛋白(SPI)-壳聚糖(CS)复合物中的α-螺旋和β-片含量分别降低到18.01%和28.67%,而紫外吸收峰则随着电场强度的增加而增加。当电场强度为 6 V/cm 时,SPI-CS 复合物的乳化活性指数和乳化稳定性指数分别为 95.52 m2/g 和 55.60 min。发泡能力和发泡稳定性分别为 148.33 % 和 115.59 %,溶解度也高达 91.37 %。此外,空气/水界面特性也有显著提高。经 OH 处理后,复合物的功能特性明显增强。上述论述为实施 OH 处理提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of ohmic heating-assisted glycation reaction on the properties of soybean protein isolate-chitosan complexes.

The purpose of this study was to investigate the progress of glycation reaction reactions by conventional heating and ohmic heating (OH) treatment, and the effect of different electric field intensities on the structure, physical and chemical and functional properties of glycosylated proteins. The findings demonstrated that OH treatment was more efficacious than conventional heating in reducing the free amino group and increasing the absorbance at 420 nm. Concurrently, the α-helix and β-sheet content of soy protein isolate (SPI)-chitosan (CS) complexes exhibited a reduction to 18.01 % and 28.67 %, respectively, while the UV absorption peak demonstrated an increase in conjunction with the escalation of electric field intensity. When the electric field intensity was 6 V/cm, the emulsification activity index and emulsion stability index of SPI-CS complexes were found to be 95.52 m2/g and 55.60 min, respectively. The foaming capacity and foaming stability were found to be 148.33 % and 115.59 % respectively, while the solubility was also up to 91.37 %. Additionally, the air/water interface properties demonstrated a notable enhancement. The functional properties of the complexes were demonstrably enhanced following the application of an OH treatment. The aforementioned statement provided a theoretical foundation for the implementation of OH treatment.

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来源期刊
International Journal of Biological Macromolecules
International Journal of Biological Macromolecules 生物-生化与分子生物学
CiteScore
13.70
自引率
9.80%
发文量
2728
审稿时长
64 days
期刊介绍: The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.
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