Cristina Romero-Aranda, Beatriz Sáenz-Narciso, Eva Gómez-Orte, Ángela Metola, Begoña Ezcurra, Olga Calvo, Hilde Nilsen, Antonio Miranda-Vizuete, Juan Cabello
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引用次数: 0
摘要
秀丽隐杆线虫 Integrator 复合物由至少 13 个进化保守的蛋白质组成，它们结合 RNA 聚合酶 II 的 C 端结构域来调控 snRNA 3'-end 处理和基因表达。在这里，我们发现整合子亚基 6 介入了秀丽隐杆线虫的 DNA 损伤反应。我们发现，在 X 射线辐射下，INTS-6 是 RAD-51 病灶形成的必要条件。此外，CDK-1 Tyr-15 磷酸化依赖于 INTS-6 的存在。这项工作为阐明整合子复合体在 DNA 修复中的作用机制增添了新的内容。
Integrator complex subunit 6 (INTS-6) mediates DNA damage response in Caenorhabditis elegans.
The Caenorhabditis elegans Integrator complex is a set of at least 13 evolutionarily conserved proteins that binds the C-terminal domain of RNA polymerase II to regulate snRNA 3'-end processing and gene expression. Here we show that the Integrator subunit 6 intervenes in the DNA damage response in C. elegans . We find that upon X-ray radiation, INTS-6 is necessary for RAD-51 foci formation. In addition, CDK-1 Tyr-15 phosphorylation depends on the presence of INTS-6 . This work adds a new piece to elucidate the Integrator complex mechanism of action in DNA repair.
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