用于跟踪人类多能干细胞及其分化后代细胞周期动态的诱导型 iFUCCI 报告细胞系。

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-11-17 DOI:10.1016/j.scr.2024.103614

Merve Ay , Desi Veleva , Mohammad Chowdhury , Sonja Drljaca , Thais Sobanski , Andrew B.J. Prowse , Dmitry A. Ovchinnikov

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引用次数: 0

摘要

实时监测细胞周期进展的能力在以多能干细胞为基础的模型和疗法开发中有着广泛的应用。基于荧光泛素化的细胞周期指示剂（FUCCI）系统能以非侵入方式实时监测干细胞及其分化后代的细胞周期。我们描述了在人类胚胎干细胞（hESC）系 MEL-1 中生成的具有强力霉素诱导报告系统 iFUCCI 的细胞系及其特征。MEL-1 iFUCCI hESC表现出正常的核型，表达未分化状态的标记物，并通过产生来自所有三个胚层的细胞群表现出预期的分化潜力。

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An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny

An ability to monitor cell cycle progression in real time has numerous applications in pluripotent stem cell-based models and therapeutics development. Fluorescence Ubiquitination-based Cell Cycle Indicator (FUCCI) systems enable live monitoring of the cell cycle in stem cells and their differentiated progenies in a non-invasive manner. We describe the generation and characterisation of a cell line with a doxycycline-inducible reporter system, iFUCCI, in the human embryonic stem cell (hESC) line MEL-1. MEL-1 iFUCCI hESCs exhibited a normal karyotype, expressed markers of the undifferentiated state, and demonstrated expected differentiation potential by giving rise to cell populations from all three germ layers.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.